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Are you having trouble with your real-time PCR experiment? Do you need help diagnosing your experiment? The real-time PCR doctor is here to help. Unexpected fluorescence data are symptomatic of problems with your real-time PCR reaction components or amplification protocol. Click one of the symptoms below to learn about possible causes and treatments.
Related Topics: What Is Real-Time PCR?, How Real-Time PCR Works, and qPCR Assay Design and Optimization.
My experiment displays:
If you don't see your symptom listed above, please contact technical support at 1-800-424-6723, option 2.
Call us at 1-800-4-BIORAD (1-800-424-6723)