Prepacked Bio-Scale™ Mini Size Exclusion Cartridges

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Bio-Scale™ Mini Bio-Gel^® P-6 Desalting Cartridges

Overview

Cartridges are prepacked with Bio-Gel P polyacrylamide gels for gel filtration to separate molecules by size. Ready to use cartridges are designed for use with BioLogic systems or any chromatography system.

Bio-Scale Mini Cartridge Selection Guide

Bio-Scale™ Mini Cartridge Selection Guide

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	5 x 1 ml	1 x 5 ml	5 x 5 ml
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	–N⁺(CH₃)₃	≥37 mg BSA	732-4120	732-4122	732-4124
UNOsphere™ Q	UNOsphereQ	Strong anion exchange	–N⁺(CH₃)₃	180 mg BSA	732-4100	732-4102	732-4104
UNOsphere S	UNOsphere S	Strong cation exchange	–SO₃^-	60 mg human lgG	732-4110	732-4112	732-4114
DEAE	Macro-Prep DEAE	Weak anion exchange	–HN⁺(C₂H₅)₂	>35 mg protein	732-4140	732-4142	732-4144
Protein Purification
High S	Macro-Prep High S	Strong cation exchange	–SO₃^-	≥49 mg human lgG	732-4130	732-4132	732-4134
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum	—	732-4632	732-4634
Antibody Purification
Protein A	UNOsphere SUPrA™	Affinity	Recombinant protein A	30 mg/ml human IgG	732-4200, 1 x 1 ml	732-4201, 5 x 1 ml	732-4202, 1 x 5 ml
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal lgG_1; 16–23 mg human lgG	732-4600	732-4602	—
CHT Type I	CHT™ ceramic hydroxyapatite Type I, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg human lgG	—	732-4322	732-4324
CHT Type II	CHT ceramic hydroxyapatite Type II, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	15–25 mg human lgG	—	732-4332	732-4334
Affinity-Tagged Protein Purification
IMAC	Profinity™ IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant histidine (His)-tagged protein	732-4610	732-4612	732-4614
GST	Profinity GST	Affinity	Glutathione	≥10 mg/ml recombinant GST-tagged protein	732-4620	732-4622	732-4624
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel Blue	Affinity	Cibacron Blue F3GA	0.2 ml serum 11 mg albumin	—	732-4642	732-4644

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
					1 x 5 ml	5 x 5 ml	5 x 10 ml	1 x 50 ml	5 x 50 ml
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel filtration	Polyacrylamide	10–22% CV**	732- 4502	732- 4504	732- 5304	732- 5312	732- 5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

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Cartridge Selection by application

Bio-Scale Mini Cartridge Specifications

Properties of Bio-Gel P Polyacrylamide Gel

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Bio-Scale™ Mini Bio-Gel^® P-6 Desalting Cartridges
These columns are 5 ml, 10 ml, and 50 ml Bio-Scale Mini cartridges filled with Bio-Gel P-6 support. They are used for desalting or buffer exchange using gel filtration (size exclusion) chromatography.

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Cartridge by Application	Support	Type of Separation	Chemical Form	Binding Capacity

Protein and Plasmid Purification
UNOSphere Rapid S media	UNOSphere Rapid S	Strong cation exchange	SO₃-	* Human IgG: 60 mg/ml
CFT media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
Apatite purification kit	Ceramic hydroxyapatite Ceramic fluoropatite	Hydroxyapatite Fluoropatite	(Ca₅(PO₄)₃OH)₂ Ca₁₀(PO₄)₆F₂	** Human IgG; 25-60 mg/ml Lysozyme 15–21 mg/g
Protein Purification
UNOSphere Rapid S media	UNOSphere Rapid S	Strong cation exchange	SO₃-	* Human IgG: 60 mg/ml
CFT media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
Apatite purification kit	Ceramic hydroxyapatite Ceramic fluoropatite	Hydroxyapatite Fluoropatite	(Ca₅(PO₄)₃OH)₂ Ca₁₀(PO₄)₆F₂	** Human IgG; 25–60 mg/ml Lysozyme 15–21 mg/g
Antibody Purification
CFT Media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
UNOsphere SUPrA media	UNOsphere SUPrA	Affinity	Protein A
Monoclonal Antibody Purification
mAb purification kit	UNOsphere SUPrA CHT Type 1 UNOsphere Q	Affinity Hydroxyapatite Strong anion exchange	Protein A (Ca₅(PO₄)₃OH)₂ -N(CH₃)₃*	** Human IgG: 25–60 mg/ml * BSA 180mg/ml


Sizes	1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate	Varies for each support type*
Maximum operating pressure	1, 5, and 10 ml: 45 psi 50 ml: 40 psi
Fittings	Female luer inlet and male luer outlet
Column material	Polypropylene
Frit material	Polyethylene (HDPE)
Dimensions	1 ml — 40 mm length x 5.6 mm inner diameter 5 ml — 40 mm length x 12.6 mm inner diameter 10 ml — 80 mm length x 12.6 mm inner diameter 50 ml — 80 mm length x 28.2 mm inner diameter


	Hydrated Bead Size (µm)	Hydrated Bed Volume Mass Dry Gel	Typical Flow Rate (cm/hr)*	Molecular Weight Fractionation Range

Bio-Gel P–2, fine	45–90	3 ml/g	5–10	100–1,800
Bio-Gel P–2, extra fine	<45		<10
Bio-Gel P–4, medium	90–180	4 ml/g	15–20	800–4,000
Bio-Gel P–4, fine	45–90		10–15
Bio-Gel P–4, extra fine	<45		<10
Bio-Gel P–6, medium	90–180	6.5 ml/g	15–20	1,000–6,000
Bio-Gel P–6, fine	45–90		10–15
Bio-Gel P–6, extra fine	<45		<10
Bio-Gel P–6DG gel	90–180		15–20
Bio-Gel P–10 gel, medium	90–180	7.5 ml/g	15–20	1,500–20,000
Bio-Gel P–10 gel, fine	45–90		10–15
Bio-Gel P–30 gel, medium	90–180	9 ml/g	7–13	2,400–40,000
Bio-Gel P–30 gel, fine	45–90		6–11
Bio-Gel P–60 gel, medium	90–180	11 ml/g	4–6	3,000–60,000
Bio-Gel P–60 gel, fine	45–90		3–5
Bio-Gel P–100 gel, medium	90–180	12 ml/g	4–6	5,000–100,000
Bio-Gel P–100 gel, fine	45–90		3–5

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Prepacked Bio-Scale™ Mini Size Exclusion Cartridges

Overview

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Prepacked Bio-Scale™ Mini Size Exclusion Cartridges

Overview

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