Macro-Prep HIC Media

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Macro-Prep Methyl HIC Media
Macro-Prep t-Butyl HIC Media

Overview

Methacrylate-based beads for protein and nucleic acid purification using hydrophobic interactions. Methyl and t-butyl supports are available for purifying compounds that have weak or strong hydrophobic regions.

Characteristics of Macro-Prep HIC Media

Chemical Structure

Macro-Prep HIC Media

Dynamic Binding Capacity

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Macro-Prep Methyl HIC Media
Macro-Prep hydrophobic interaction chromatography (HIC) media are methacrylate-based, 50 μm beads used for protein, polypeptide, enzyme, and nucleic acid purification.

Macro-Prep t-Butyl HIC Media
Macro-Prep hydrophobic interaction chromatography (HIC) media are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification.

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	Methyl HIC Support	t-Butyl HIC Support
Functional group	-CH₃	-C(CH₃)₃
Nominal particle size	50 µm	50 µm
Binding capacity*	>25 mg/ml	>15 mg/ml
Recommended maximum linear flow rate	3,000 cm/hr	3,000 cm/hr
pH stability	1–12	1–12
Regeneration	70% ethanol	70% ethanol
Autoclavability (121°C, 30 min)	Yes	Yes
Storage	20% ethanol	20% ethanol

		Retention Times (min)
Peak	Protein	Methyl	t-Butyl
1	Cytochrome c	7.3	10.41
2	Ovalbumin	32.25	40.22
3	Α-Anylase	62.81	71.5
4	Ferritin	81.49	90.9

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Macro-Prep HIC Media

Overview

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Macro-Prep HIC Media

Overview

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