 |  |  |
| Operating Limits |
| Flow rate | 40 ml/min; optional F40 pump kit
80 ml/min; optional BioLogic Maximizer valve system | 20 ml/min per channel; dual-channel peristaltic pump | 20 ml/min per channel; dual-channel peristaltic pump |
| Pressure | 3,500 psi (23 MPa) at 10 ml/min (F10 pumps)
1,000 psi (6.6 MPa) at 40 ml/min (F40 pumps) | 30 psi (0.2 MPa) | 30 psi (0.2 MPa) |
| Detection |
| UV detection | Standard 254 and 280 nm filters; optional 214, 365, 405, and 436 nm filters; standard 5 mm analytical flow cell; optional 2 mm preparative flow cell; optional BioLogic QuadTec UV/Vis detector for simultaneous 4-wavelength detection | 254 and 280 nm filters; 2 mm flow cell | Optional Model EM-1 Econo UV monitor; 254 and 280 nm filters |
| Conductivity | Standard | Standard | Optional Model EG-1 Econo gradient monitor |
| pH | Standard (BioLogic DuoFlow Maximizer, Biologic DuoFlow PathFinder systems)
Optional (BioLogic DuoFlow, BioLogic QuadTec systems) | — | — |
| Other | Optional signal import module (SIM) adds pH, refractive index, and fluorescence detectors | — | — |
| Sample Loading |
| Large samples/buffer selection | Optional SV5-4 valve, Model EP-1 Econo pump or Econo gradient pump | Optional SV-5 valve | Optional SV3-2 valve |
| Single sample | AVR7-3 valve | Manual MV-6 valve | Optional MV-6 valve |
| Multiple samples | Optional DynaLoop sample loops (25 ml, 90 ml); Econo gradient pump or EP-1 Econo pump with AVR9-8 valve | Optional SV-5 valve | — |
| Fraction Collection |
| General | BioFrac or Model 2110 fraction collector | BioFrac or Model 2110 fraction collector | BioFrac or Model 2110 fraction collector |
| Large-volume | BioFrac fraction collector with optional micro tube adaptor or AVR9-8 valve | BioFrac fraction collector with optional micro tube adaptor | BioFrac fraction collector with optional micro tube adaptor |
| Small-volume | BioFrac or Model 2110 fraction collector | BioFrac or Model 2110 fraction collector | BioFrac or Model 2110 fraction collector |
| Special Applications |
| Buffer blending | BioLogic Maximizer valve system (BioLogic DuoFlow Maximizer, BioLogic DuoFlow Pathfinder systems); optional in BioLogic DuoFlow, BioLogic QuadTec systems | — | — |
| Column scouting/column switching | Optional AVR9-8 valves for ≤8 columns or additional AVR7-3 valve for 2 columns | — | — |
| Reverse flow | Additional AVR7-3 valve | — | — |
| Solvent Compatibility | All commonly used chromatographic solvents | All commonly used chromatographic solvents | All commonly used chromatographic solvents |
| Operating Temperature | 4–40°C | 4–40°C | 4–40°C |
| Comments | Flexible modular design, easily upgraded.
High resolution, suitable for analytical and preparative chromatography.
Intuitive, easy-to-learn software. | Flexible modular design.
Ideal for initial purification, purification of large volumes of crude sample, or affinity separation of recombinant proteins.
Intuitive software; PC can be used as chart recorder. | Expandable with peripherals and accessories.
Stand-alone pump for isocractic and gradient elution in preparative applications.
Control of optional splitter valve.
Integratable with BioLogic DuoFlow software for large-volume sample loading. |
| Dimensions (W x D x H) | 35 x 41 x 81 cm (13.8 x 16.1 x 31.9") | 24 x 33 x 25 cm (9.5 x 13.0 x 9.8") | 14 x 20 x 20 cm (5.5 x 7.9 x 7.9") |
| Weight | 70 kg (154 lb) | 19 kg (42 lb) | 3.5 kg (7.7 lb) |
