Probe Cleanup Spin Columns

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Overview

Prepacked size exclusion spin columns, using specially sized Bio-Gel polyacrylamide gels, allow for easy cleanup and purification of radiolabeled probes, nick translations, plasmid, and PCR reaction mixtures.

Specifications

Column Selection Guide

PCR/DNA Purification Guide

Category Products

Micro Bio-Spin^® Columns With Bio-Gel^® P-6
Micro Bio-Spin columns filled with Bio-Gel P-6 gels clean nucleic acid samples quickly using size exclusion chromatography.

Micro Bio-Spin^® Columns With Bio-Gel^® P-30
Micro Bio-Spin columns filled with Bio-Gel P-30 gels clean nucleic acid samples quickly using size exclusion chromatography.

Bio-Spin^® Columns With Bio-Gel^® P-6
Bio-Spin columns filled with Bio-Gel P-6 gels clean nucleic acid samples quickly using size exclusion chromatography.

Bio-Spin^® Columns With Bio-Gel^® P-30
Bio-Spin columns filled with Bio-Gel P-30 gels clean nucleic acid samples quickly using size exclusion chromatography.

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	Bio-Spin 6	Micro Bio-Spin 6	Bio-Spin 30	Micro Bio-Spin 30
Packed support	Special grade Bio-Gel P-6 gel	Special grade Bio-Gel P-6 gel	Special-grade Bio-Gel P-30 gel	Special-grade Bio-Gel P-30 gel
Equilibration buffer	SSC-buffer*	10 mMTris, pH 7.4 or SSC buffer*	SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*
Applications	Desalting and buffer exchange	Desalting and buffer exchange	Desalting; nucleotide and small molecule removal	DNA sequencing reaction mixtures (Tris), and small molecule removal
Bed volume	1.1 ml	0.7 ml	1.1 ml	0.7 ml
Retention and recovery	90% recovery of 20 bases or bp, 99% retention of salts	90% recovery of 20 bases or bp, 99% retention of salts	95% recovery of 22 bases or bp, 98% retention of ddNTPs	95% recovery of 22% bases or bp, 98% retention of ddNTPs
MW exclusion limit, globular proteins	6,000	6,000	40,000	40,000
Sample volume	50–100 µl	10–75 µl	50–100 µl	10–75 µl
Centrifuge type	Swinging bucket	Microcentrifuge	Swinging bucket	Microcentrifuge
Autoclavability	Yes	Yes	Yes	Yes

	Bio-Spin 6	Micro Bio-Spin 6	Bio-Spin 30	Micro Bio-Spin 30	PCR Kleen
Packed support	Special grade Bio-Gel P-6 gel	Special grade Bio-Gel P-6 gel	Special grade Bio-Gel P-30 gel	Special grade Bio-Gel P-30 gel	Special grade Size exclusion gel
Equilibration buffer	10 mM Tris, pH 7.4 or SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*	10 mM Tris, 1 mM EDTA, pH 7.0
Applications Desalting of oligonucleotides >20 bases	•	•	-	-	-
Labeling reactions: removal of unincorporated nucleotides >20 bases or bp from DNA	-	-	•	•	-
Removal of primers and primer-dimers from PCR products >200 bp	-	-	-	-	•
Buffer exchange (restriction fragments, PCR products, enzyme reactions, sequencing templates.)	•	•	-	•	-
DNA sequencing reaction mixture cleanup**	-	-	•	•	-
Riboprobe cleanup***	-	-	-	•	-
Desalting of antibody, enzyme, and protein solutions	•	•	-	•	-
Purification of proteins of moleular weight >6,000	•	•	-	-	-
Purification of proteins of molecular weight >40,000	-	-	•	•	-
Bed Volume	1.1 ml	0.7 ml	1.1 ml	0.7 ml	0.6 ml
Retention and recovery	90% recovery of 20 bases or bp, 99% retention of salts	90% recovery of 20 bases or bp, 99% retention of salts	95% recovery of 22 bases or bp, 98% retention of ddNTPs	95% recovery of 22 bases or bp, 98% retention of ddNTPs	85% recovery of ≥700bp, 95% retention of primers and primer-dimers
Molecular weight exclusion limit, globular proteins	6,000	6,000	40,000	40,000	8,000,000
Sample volume	50–100 µl	10–75 µl	50–100 µl	10–75 µl	25–100 µl
Centrifuge type	Swinging bucket	Microcentrifuge	Swinging bucket	Microcentrifuge	Microcentrifuge
Autoclavability	Yes	Yes	Yes	Yes	Yes

	Freeze 'N Squeeze™ DNA Gel Extraction Spin Columns	Micro Bio-Spin^® and Bio-Spin Columns	PCR Kleen™ Spin Columns
Application	Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels	Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting	Removal of PCR products >200 bp from other reaction mixture components
Starting material	≤700 mg gel slice	10–100 µl of sequencing reation mixture	25–100 µl of PCR or other enzyme reaction
Format	Spin-column extraction of frozen-thawed gel slices	Prepacked size exclusion spin column	Prepacked size exclusion spin column
Recovery	4–23 kb: >80% recovery; 0.5–4 kb:>70% recovery; 50–500 bp:>50% recovery	>95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators	50–80%
Time required	<10 min	<10 min	<4 min

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Probe Cleanup Spin Columns

Overview

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Probe Cleanup Spin Columns

Overview

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