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Electrophoresis buffers and reagents are available as individual reagents or premixed buffers. Products include loading buffers for protein or nucleic acid samples, detergents, ampholytes, and gel-casting buffers.
Electrophoresis Buffers With premixed electrophoresis running buffers, you standardize your electrophoresis runs and save on preparation time, while avoiding mistakes in buffer concentration. Bio-Rad buffers are made with high-purity water and our own pure reagents, and are 0.4 µm filtered, ensuring the highest quality. Premixed buffers are available for a variety of protein and nucleic acid electrophoresis protocols. Our 5 L boxes offer tremendous economical and convenience advantages. They are compact and stackable to save benchspace, and are designed with an easy-pour spout.
Blot Processing Buffers The processing of blots for protein and nucleic acid detection is now even simpler with a variety of premixed wash buffers and blocking solutions.
Electrophoresis Buffer Reagents In case you would like to prepare it all yourself, we offer a complete line of reagents. Our classic electrophoresis powder reagents are the ultimate in high quality.
Blot Transfer Buffers The transfer buffer must facilitate both effective elution from the gel matrix and effective binding of the protein or nucleic acid to the membrane. Determine your choice of buffer by the type of gel or membrane and the physical characteristics of the molecules of interest.
Sample Loading Buffers Premixed loading buffers remove variables that cause lane-to-lane running anomalies, and since no preparation is required, you save valuable time as well. Bio-Rad premixed sample buffers are available for numerous applications, including native PAGE, SDS-PAGE, peptide analysis, analytical IEF, nucleic acid sample preparation (denaturing and nondenaturing), and zymogram gel sample preparation.
SDS Solutions Detergents are employed in electrophoresis when it is necessary to disrupt protein-lipid or protein-protein interactions. SDS is the most common detergent used in PAGE analysis because most proteins are readily soluble in it. Bio-Rad SDS solutions are highly purified — an important feature, since impurities in SDS have unpredictable effects on electrophoretic mobilities.
Gel Casting Solutions Tris Buffers and Acrylamide Solutions for Gel Casting Bio-Rad offers a variety of prepared solutions for casting polyacrylamide gels. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels, according to Laemmli or Ornstein-Davis discontinuous buffer systems. Acrylamide solutions are provided ready to use and come with instructions. High-purity reagents and carefully controlled manufacturing conditions allow acrylamide solutions to be stable for 1 year at 4°C.
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