Buffers and Reagents for Protein Electrophoresis

Electrophoresis Buffers
With premixed electrophoresis running buffers, you standardize your electrophoresis runs and save on preparation time, while avoiding mistakes in buffer concentration. Bio-Rad buffers are made with high-purity water and our own pure reagents, and are 0.4 µm filtered, ensuring the highest quality. Premixed buffers are available for a variety of protein and nucleic acid electrophoresis protocols. Our 5 L boxes offer tremendous economical and convenience advantages. They are compact and stackable to save benchspace, and are designed with an easy-pour spout.

Blot Processing Buffers
The processing of blots for protein and nucleic acid detection is now even simpler with a variety of premixed wash buffers and blocking solutions.

• Premixed blocking buffers, available as TBS/casein and PBS/casein, take the time and effort out of solubilizing casein
• Premixed wash buffers in TBS, PBS, and SSC reduce the number of stock solutions to prepare
• 10% Tween 20 makes pipetting accurate and simple

Electrophoresis Buffer Reagents
In case you would like to prepare it all yourself, we offer a complete line of reagents. Our classic electrophoresis powder reagents are the ultimate in high quality.

Blot Transfer Buffers
The transfer buffer must facilitate both effective elution from the gel matrix and effective binding of the protein or nucleic acid to the membrane. Determine your choice of buffer by the type of gel or membrane and the physical characteristics of the molecules of interest.

Sample Loading Buffers
Premixed loading buffers remove variables that cause lane-to-lane running anomalies, and since no preparation is required, you save valuable time as well. Bio-Rad premixed sample buffers are available for numerous applications, including native PAGE, SDS-PAGE, peptide analysis, analytical IEF, nucleic acid sample preparation (denaturing and nondenaturing), and zymogram gel sample preparation.

SDS Solutions
Detergents are employed in electrophoresis when it is necessary to disrupt protein-lipid or protein-protein interactions. SDS is the most common detergent used in PAGE analysis because most proteins are readily soluble in it. Bio-Rad SDS solutions are highly purified — an important feature, since impurities in SDS have unpredictable effects on electrophoretic mobilities.

Gel Casting Solutions
Tris Buffers and Acrylamide Solutions for Gel Casting
Bio-Rad offers a variety of prepared solutions for casting polyacrylamide gels. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels, according to Laemmli or Ornstein-Davis discontinuous buffer systems. Acrylamide solutions are provided ready to use and come with instructions. High-purity reagents and carefully controlled manufacturing conditions allow acrylamide solutions to be stable for 1 year at 4°C.