Low-Pressure Chromatography Columns and Cartridges

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Overview

These prepacked and empty columns are available for use with low-pressure chromatography systems, such as the BioLogic LP system, and the Profinia protein purification system.

Prepacked Cartridge Selection Guide

Connecting a Flow Adaptor

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Affinity Bio-Scale™ Mini Cartridges
Bio-Scale Mini cartridges are prepacked with various media for purification of proteins by affinity chromatography. Luer fittings enable connection to any chromatography system, or elution using a ...More »

Ion Exchange Bio-Scale™ Mini Cartridges
These Bio-Scale Mini cartridges are prepacked with various media for ion exchange chromatography. Luer fittings enable connection to any chromatography system, or elution using a Luer-Lok syringe.

Bio-Scale™ Mini CHT™ 40 µm Cartridges
Bio-Scale Mini CHT cartridges are 5 ml Bio-Scale Mini cartridges filled with either CHT Type I or Type II having a particle size of 40 µm.

Bio-Scale™ Mini Bio-Gel P-6 Desalting Cartridges
These columns are 5 ml, 10 ml, and 50 ml Bio-Scale Mini cartridges filled with Bio-Gel P-6 support. They are used for desalting or buffer exchange using gel filtration (size exclusion) chromatography.

Glass Econo-Column Columns
Econo-Column chromatography columns are the standard for high-quality, affordable low-pressure chromatography columns. Columns ranging from 5 to 170 cm long and 0.5 to 5.0 cm in diameter are available.

Glass Econo-Column Accessories
Reservoirs are available in 500 ml and 1 L capacities and will fit 0.5, 0.7, 1.0, or 1.5 cm ID Econo-Column chromatography columns.

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Cartridge by Application	Support	Type of Separation	Chemical Form	Protein capacity/ml*
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	-N⁺(CH₃)₃	≥37 mg BSA
UNOsphere Q	UNOsphere™ Q	Strong anion exchange	-N⁺(CH₃)₃	180 mg BSA
UNOsphere S	UNOsphere S	Strong cation exchange	-SO₃^-	60 mg human IgG
DEAE	Macro-Prep DEAE	Weak anion exchange	-HN⁺(C₂H₅)₂	>35 mg protein
Protein Purification
High S	Macro-Prep High S	Strong cation exchange	-SO₃^-	≥49 mg human IgG
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum
Antibody Purification
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal IgG₁
CHT™ Type I	CHT ceramic hydroxyapatite Type I	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	16–23 mg human IgG
CHT Type II	CHT ceramic hydroxyapatite Type II	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC**	Profinity™ IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant His-tagged protein
GST**	Profinity GST	Affinity	Glutathione	≥10 mg/ml recombinant GST-tagged protein
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel filtration	Polyacrylamide	10–22% CV***
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel Blue	Affinity	Cibacron Blue F3GA	0.2 mlm serum 11 mg albumin

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Low-Pressure Chromatography Columns and Cartridges

Overview

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Low-Pressure Chromatography Columns and Cartridges

Overview

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