Polyacrylamide is a commonly used electrophoresis matrix formed by free-radical polymerization of acrylamide and a co-monomer crosslinker. Reliable electrophoresis results depend on the reproducibility of this reaction, making highly-purified starting reagents an important factor in gel quality. For a discussion of the factors affecting acrylamide polymerization, request bulletin 1156.
Pure Acrylamide
Bio-Rad's 99.9% pure acrylamide undergoes rigorous purification and testing to ensure the quality necessary for uniform polymerization. It is available in four different configurations to meet the needs of every lab.
Acrylamide Powders
Acrylamide and bis-acrylamide powders allow complete adjustment of concentration and acrylamide/bis ratio, to meet all application needs. Powders are available in a variety of package sizes, are economical, and have a shelf life of 1 year or more. They can be stored at room temperature, saving cold-storage space.
Acrylamide/Bis Solutions
Bio-Rad's acrylamide/bis solutions are made from 99.9% pure acrylamide and bis-acrylamide and are available in two concentrations, 30% and 40%, and three different crosslinker ratios, 19:1, 29:1 and 37.5:1. Acrylamide/bis solutions are provided ready to use and come with instructions. High-purity reagents and carefully controlled manufacturing conditions allow acrylamide/bis solutions to be stable for 1 year at 4°C.
Premixed Acrylamide/Bis Powders
Premixed acrylamide/bis powders contain premeasured amounts of Bio-Rad's 99.9% pure acrylamide and bis-acrylamide. Stock solutions from 30% to 50% can be prepared directly in the bottle by adding the indicated amount of water and stirring. The shelf life of the premixed powders is 1 year at room temperature. After they have been made into solutions, premixed powders should be stored at 4°C, away from light, for maximum stability.
