Bio-Plex^® Cell Signaling Assays

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Bio-Plex Pro™ Magnetic Cell Signaling Assays
Bio-Plex^® Nonmagnetic Signal Transduction Assays

Overview

Assays for the detection of multiple analytes involved in intracellular cell signaling pathways. Magnetic and nonmagnetic singleplex, multiplex, and custom configurations.

Available Magnetic Assays

Available Nonmagnetic Assays

Analyte Guide

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Bio-Plex Pro™ Magnetic Cell Signaling Assays
Bio-Plex Pro cell signaling assays are magnetic bead–based immunoassays for the detection of intracellular phosphoproteins and total target proteins in cell and tissue lysates.

Bio-Plex^® Nonmagnetic Signal Transduction Assays
Bio-Plex phosphoprotein assays are bead-based multiplexable assays that directly detect phosphorylated proteins in lysates derived from cell culture or tissue samples. Using these 96-well...

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Phosphoprotein Assays		Total Protein Target Assays		Bead Region
Akt (Ser⁴⁷³)	171-V50001M	Akt	171-V60001M	75
Akt (Thr³⁰⁸)	171-V50002M	Akt	171-V60001M	75
c-Jun (Ser⁶³)	171-V50003M	c-Jun	171-V60002M	56
EGFR (Tyr¹⁰⁶⁸)	171-V50004M			44
EGFR (Tyr¹¹⁷³)	171-V50005M			44
Erk1/2 (Thr²⁰²/Tyr²⁰⁴, Thr¹⁸⁵/Tyr¹⁸⁷)	171-V50006M	Erk 1/2	171-V60003M	38
GSK-3α/β (Ser²¹/Ser⁹)	171-V50007M	GSK-3β	171-V60004M	18
HER-2 (Tyr¹²⁴⁸)	171-V50008M	HER-2	171-V60005M	30
IGF-IR (Tyr¹¹³¹)	171-V50009M			43
IκB-α (Ser³²/Ser³⁶)	171-V50010M	IκB-α	171-V60006M	67
JNK (Thr¹⁸³/Tyr¹⁸⁵)	171-V50011M	JNK	171-V60007M	34
MEK1 (Ser²¹⁷/Ser²²¹)	171-V50012M	MEK1	171-V60008M	27
NF-κB p65 (Ser⁵³⁶)	171-V50013M			37
p38 MAPK (Thr¹⁸⁰/Tyr¹⁸²)	171-V50014M	p38 MAPK	171-V60009M	36
p70 S6 Kinase (Thr⁴²¹/Ser⁴²⁴)	171-V50015M	p70 S6 Kinase	171-V60010M	55
p70 S6 Kinase (Thr³⁸⁹)	171-V50016M	p70 S6 Kinase	171-V60010M	55
PDGFR-α (Tyr⁷⁵⁴)	171-V50017M			28
PDGFR-β (Tyr⁷⁵¹)	171-V50018M			57
Smad2 (Ser⁴⁶⁵/Ser⁴⁶⁷)	171-V50019M	Smad2	171-V60011M	14
Stat1 (Tyr⁷⁰¹)	171-V50020M			61
Stat3 (Ser⁷²⁷)	171-V50021M			52
Stat3 (Tyr⁷⁰⁵)	171-V50022M			52
VEGFR-2 (Tyr¹¹⁷⁵)	171-V50023M			29

Assays	Phosphoprotein	Total	Bead Region
Akt Signaling
Akt (Ser⁴⁷³)	•	•	75
GSK-3α/β (Ser²¹/Ser⁹)	•	—	18
Cell Cycle/Checkpoint Control
p53 (Ser¹⁵)	•	•	53
p53 (Ser³⁷)	•	•	53
p53 (Ser⁴⁶)	•	•	53
Chromatin Regulation/Acetylation
Histone H3 (Ser¹⁰)	•	—	77
Immunology/Inflammation
IκB-α (Ser³²/Ser³⁶)	•	•	58
NF-κB p65 (Ser⁵³⁶)	•	—	37
STAT2 (Tyr⁶⁸⁹)	•	—	32
STAT3 (Ser⁷²⁷)	•	—	52
STAT3 (Tyr⁷⁰⁶)	•	—	52
STAT6 (Tyr⁶⁴¹)	•	—	72
Tyk2 (Tyr¹⁰⁵⁴/Tyr¹⁰⁵⁵)	•	—	33
Glucose/Energy Metabolism
IGF-IR (Tyr¹¹³¹)	•	—	43
IR-β (Tyr¹¹⁴⁶)	•	—	43
IRS-1 (Ser⁶³⁶/Ser⁶³⁹)	•	—	76
MAP Kinase Signaling
ATF-2 (Thr⁷¹)	•	•	20
c-Jun (Ser⁶³)	•	•	56
ERK1 (Thr²⁰²/Tyr²⁰⁴)	•	—	38
ERK2 (Thr¹⁸⁵/Tyr¹⁸⁷)	•	•	38
ERK1/2 (Thr²⁰²/Tyr²⁰⁴, Thr¹⁸⁵/Tyr¹⁸⁷)	•	•	38
HSP27 (Ser⁷⁸)	•	•	51
JNK (Thr¹⁸³/Tyr¹⁸⁵)	•	•	34
MEK1 (Ser²¹⁷/Ser²²¹)	•	•	40
p38 MAPK (Thr¹⁸⁰/Tyr¹⁸²)	•	•	36
p90RSK (Thr³⁵⁹/Ser³⁶³)	•	•	35
Neuroscience
CREB (Ser¹³³)	•	•	19
TrkA (Tyr⁴⁹⁰)	•	—	73
Translational Control
p70 S6 kinase (Thr⁴²¹/Ser⁴²⁴)	•	—	55
S6 ribosomal protein (Ser²³⁶/Ser²³⁶)	•	—	74
Tyrosine Kinases
Bcr-AbI (Tyr²⁴⁵)	•	•	45
c-AbI (Tyr²⁴⁵)	•	—	45
c-AbI (Tyr⁴¹²)	•	—	45
EGFR (Tyr)	•	—	20
PDGF receptor-β (Tyr⁷⁵¹)	•	—	57
Src (Tyr⁴¹⁶)	•	—	41

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Bio-Plex^® Cell Signaling Assays

Overview

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Bio-Plex® Cell Signaling Assays

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Bio-Plex^® Cell Signaling Assays

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