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Cartridges are prepacked with Bio-Rad's UNOsphere and Macro-Prep chromatography media for ion exchange chromatography. Ready to use cartridges are designed for use with BioLogic systems or any chromatography system.
* Recommended flow rates are given for each support type in the instruction manual. ** Available for desalting cartridges only.
F3GA and DEAE
* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.** Column volume.
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* Flow rate = 150 cm/hr; ** flow rate = 300 cm/hr
Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.
Dynamic protein binding capacity as a function of linear flow rate. A 100 ml sample (3 mg.ml) of BSA was run on a 1 x 10 cm (8 ml) column at the indicated flow rates. The buffer was 50 mM Tris-HCI, pH 8.3. Elution was with 1.0 M NaCl. Read more.
Analysis of plasmid DNA purified on UNOsphere Q support. Fractions were separated on a 0.8% agarose gel. Lane 1, 1 kb marker (catalog #170-8204); lane 2, crude lysate; lane 3, flowthrough (fractions 2–6); lane 4, fractions 9–18; lane 5, fractions 19–30; lane 6, fractions 31–35; lane 7, fraction 36.
Purification of murine IgG1 on UNOsphere S column. Column size, 0.5 x 10 cm (2 ml); sample, 15 ml (6.6 mg) of murine IgG1-conditioned medium. The sample was loaded onto the column in 20 mM citrate-phosphate buffer, pH 4.0, washed, and eluted in a linear gradient of 0–100% 20 mM citrate-phosphate, pH 8.0 in 10 column volumes at a flow rate of 2.0 ml/min (600 cm/hr). Each fraction was 2.0 ml. (—), A280; (—), buffer pH.
The Macro-Prep DEAE derivative has high dynamic binding capacity and exhibits consistently high resolution even at high flow rates and high sample loads. A sample containing 3 mg/ml BSA was separated on a 1 x 10 (3 ml) Macro-Prep DEAE column. Sample was loaded onto the column in 10 mM Tris-HCI, pH 7.6 (buffer A) and was eluted in a M NaCl in buffer A. Sample was applied until the eluting buffer had an A280 value 50% of the incoming sample. Read more.
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