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Lipid transfection reagents provide optimal transfection efficiencies for nucleic acid delivery into mammalian cells. General use, cell line–specific, and RNAi-optimized transfection reagents available.
Bio-Rad offers a family of lipid transfection reagents that effectively deliver nucleic acids to a wide range of cultured mammalian cell lines, including ones that are difficult to transfect. Each lipid is optimal for certain applications. The siLentFect lipid reagent is an RNAi-specific lipid that is ideal for siRNA delivery. It can also be used to perform co-transfection with a small reporter-plasmid along with the siRNA molecule. The TransFectin lipid reagent is a general-purpose lipid that is ideal for plasmid delivery in a wide range of cell lines. The cell line-specific lipid reagents, COSFectin and HEKFectin, are best suited for experiments using the specified cell line and are particularly valuable for protein over-expression and viral production.
* siLentFect lipid reagent can successfully co-transfect an siRNA molecule with a plasmid DNA. ** Not for gene expression.*** For a more complete list of cell lines, go to www.bio-rad.com/transfection/.
The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists like you. Submit a protocol by clicking the proper technology or browse protocols to view our library and find your starting point.
Bio-Rad Laboratories has helped scientists deliver molecules into cells for over 35 years. Instruments, including the Gene Pulser® series of electroporators, the MicroPulser™ electroporator, and the Helios® gene gun and PDS-1000/He™ biolistic systems, have been used successfully to transfect prokaryotic and eukaryotic cells, including plant and animal cells. These transfection instruments are cited extensively in the literature. Our chemically mediated methods include several lipid-based transfection reagents — TransFectin™ lipid reagent for delivery of plasmid DNA, siLentFect™ lipid reagent for siRNA delivery, or HEKFectin™ and COSFectin™ lipid reagents for cell line-specific transfection.
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