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Freeze 'N Squeeze DNA
Gel Extraction Spin Columns |
Micro Bio-Spin and
Bio-Spin Columns |
PCR Kleen
Spin Columns |
| Application |
Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels |
Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting |
Removal of PCR products >200 bp from other reaction mixture components |
| Starting Material |
≤700 mg gel slice |
10–100 µl of sequencing reaction mixture |
25–100 µl of PCR or other enzyme reaction |
| Format |
Spin-column extraction of frozen-thawed gel slices |
Prepacked size exclusion spin column |
Prepacked size exclusion spin column |
| Recovery |
4–23 kb: >80% recovery;
0.5–4 kb: >70% recovery;
50–500 bp: >50% recovery |
>95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators |
50–80% |
| Time Required |
<10 min |
<10 min |
<4 min |
