Immunodetection Reagents and Kits

Immun-Star chemiluminescence detection

1. AP-conjugated secondary antibody binds to primary antibody.
2. AP (E) converts chemiluminescent substrate (S), which emits light.
3. Film or phosphor screen exposed by emitted light.

 

Deposition-based total protein stains. All proteins on the blot bind dye or gold.

Biotin-Blot total protein stain

1. N-Hydroxysuccinimido-biotin biotinylates all proteins on the blot.
2. Avidin-HRP binds to biotin.
3. HRP catalyzes reaction of 4CN substrate (S) to colored product (P).

Colorimetric Detection Chemistry.

1. Antigen-specific primary antibody binds to protein of interest.
2. Enzyme-conjugated secondary antibody or binding protein binds to primary antibody.
3. Enzyme (E) converts substrate (S) to colored precipitate (P).

Amplified Opti-4CN kit chemistry

1. Antigen-specific primary antibody binds to the protein of interest.
2. HRP-conjugated secondary antibody binds to the primary antibody.
3. Amplification reagent reacts with HRP to incorporate biotin at the protein site.
4. Streptavidin-HRP binds to the incorporated biotin.
5. Enzyme converts substrate (S) to colored precipitate (P).

Immun-Blot Amplified AP kit chemistry.

1. Biotinylated secondary antibody binds to primary antibody.
2. Complex of streptavidin and biotinylated-AP binds to biotin of secondary antibody.
3. Multiple APs are available to convert substrate (S) to colored precipitate (P).