This guidebook is a comprehensive source of information on real-time PCR. Complete with detailed information on optimizing, validating, and analyzing real-time PCR data, this technically focused applications guide is a resource for experienced researchers as well as those wanting to learn more about using real-time PCR for applications such as qPCR, RT-qPCR, genotyping, and GMO detection. This guidebook includes chapters that cover:
- Key concepts of real-time PCR
- Getting started — experimental design
- Multiplexing considerations
- Data analysis
- Gene expression analysis
- Genotyping allelic discrimination
- GMO detection
This guidebook comes with an easy-to-review real-time PCR quick guide to use at the bench. The quick guide covers the fundamentals of qPCR, design, optimization, and data analysis of qPCR experiments.
