| |
Bio-Spin 6 |
Micro Bio-Spin 6 |
Bio-Spin 30 |
Micro Bio-Spin 30 |
PCR Kleen |
| Packed support |
Special grade
Bio-Gel P-6 gel |
Special grade
Bio-Gel P-6 gel |
Special grade
Bio-Gel P-30 gel |
Special grade
Bio-Gel P-30 gel |
Special grade
Size exclusion gel |
| Equilibration buffer |
10 mM Tris, pH 7.4 or SSC buffer* |
10 mM Tris, pH 7.4 or SSC buffer* |
10 mM Tris, pH 7.4 or SSC buffer* |
10 mM Tris, pH 7.4 or SSC buffer* |
10 mM Tris, 1 mM EDTA, pH 7.0 |
| Applications Desalting of oligonucleotides >20 bases |
• |
• |
- |
- |
- |
Labeling reactions:
removal of unincorporated nucleotides >20 bases or bp from DNA |
- |
- |
• |
• |
- |
| Removal of primers and primer-dimers from PCR products >200 bp |
- |
- |
- |
- |
• |
Buffer exchange
(restriction fragments, PCR products, enzyme reactions, sequencing templates.) |
• |
• |
- |
• |
- |
DNA sequencing
reaction mixture cleanup** |
- |
- |
• |
• |
- |
| Riboprobe cleanup*** |
- |
- |
- |
• |
- |
| Desalting of antibody, enzyme, and protein solutions |
• |
• |
- |
• |
- |
| Purification of proteins of moleular weight >6,000 |
• |
• |
- |
- |
- |
| Purification of proteins of molecular weight >40,000 |
- |
- |
• |
• |
- |
| Bed Volume |
1.1 ml |
0.7 ml |
1.1 ml |
0.7 ml |
0.6 ml |
| Retention and recovery |
90% recovery of 20 bases or bp, 99% retention of salts |
90% recovery of 20 bases or bp, 99% retention of salts |
95% recovery of 22 bases or bp, 98% retention of ddNTPs |
95% recovery of 22 bases or bp, 98% retention of ddNTPs |
85% recovery of ≥700bp, 95% retention of primers and primer-dimers |
| Molecular weight exclusion limit, globular proteins |
6,000 |
6,000 |
40,000 |
40,000 |
8,000,000 |
| Sample volume |
50–100 µl |
10–75 µl |
50–100 µl |
10–75 µl |
25–100 µl |
| Centrifuge type |
Swinging bucket |
Microcentrifuge |
Swinging bucket |
Microcentrifuge |
Microcentrifuge |
| Autoclavability |
Yes |
Yes |
Yes |
Yes |
Yes |
