Selection Guide
| Precision Plus Protein™ Standards |
| |
 |
 |
 |
 |
 |
 |
Molecular weight (MW)/ pl range |
10–250 kD |
10–250 kD |
2–250 kD |
10–250 kD |
10–250 kD |
10–250 kD |
| Number of proteins |
10 |
10 |
12 |
10 |
10 |
10 |
| Electrophoresis |
|
|
|
|
|
|
| SDS-PAGE |
• |
• |
• |
• |
• |
• |
| Accurate MW estimation |
• |
• |
• |
• |
• |
• |
| Multicolored |
• |
• |
• |
• |
– |
– |
| Coomassie staining |
• |
• |
• |
• |
• |
• |
| Fluorescent staining |
– |
– |
– |
– |
– |
• |
| 2-D electrophoresis |
– |
– |
– |
– |
– |
– |
| IEF |
– |
– |
– |
– |
– |
– |
Plug format for use in gels with no reference well |
– |
– |
– |
– |
– |
– |
| Blotting |
|
|
|
|
|
|
| Monitoring transfer efficiency |
• |
• |
• |
• |
• |
– |
| Coomassie staining |
• |
• |
• |
• |
• |
• |
| Fluorescent staining |
– |
– |
– |
– |
– |
• |
| Fluorescent blotting* |
• |
• |
• |
• |
• |
– |
| Immonodetection** |
• |
– |
– |
• |
– |
• |
| Catalog Numbers |
|
|
|
|
|
|
| Single unit |
161-0385 |
161-0375 |
161-0377 |
161-0374 |
161-0373 |
161-0363 |
| Value pack of 5 units |
161-0398 |
161-0395 |
161-3097 |
161-0394 |
161-0393 |
161-0396 |
| Prestained Natural Standards |
| |
 |
 |
 |
 |
Molecular weight (MW)/ pl range |
6.9–210 kD |
14–97 kD |
45–200 kD |
7.6–216 kD |
| Number of proteins |
8 |
6 |
4 |
7 |
| Electrophoresis |
| SDS-PAGE |
• |
• |
• |
• |
| Accurate MW estimation |
– |
– |
– |
– |
| Multicolored |
– |
– |
– |
• |
| Coomassie staining |
• |
• |
• |
• |
| Fluorescent staining |
– |
– |
– |
– |
| 2-D electrophoresis |
– |
– |
– |
– |
| IEF |
– |
– |
– |
– |
Plug format for use in gels with no reference well |
– |
– |
– |
– |
| Blotting |
| Monitoring transfer efficiency |
• |
• |
• |
• |
| Coomassie staining |
• |
• |
• |
• |
| Fluorescent staining |
– |
– |
– |
– |
| Fluorescent blotting* |
– |
– |
– |
– |
| Immonodetection** |
– |
– |
– |
– |
| Catalog Numbers |
| Single unit |
161-0318 |
161-0305 |
161-0309 |
161-0324 |
| Value pack of 5 units |
– |
– |
– |
– |
| |
Unstained Natural Standards |
Specialty Standards |
| |
 |
 |
 |
 |
 |
 |
 |
Molecular weight (MW)/ pl range |
6.5–200 kD |
14–97 kD |
45–200 kD |
1.4–26.6 kD |
4.45–9.6 pl |
17.5–76 kD 4.5–8.5 pl |
10–250 kD |
| Number of proteins |
9 |
6 |
5 |
6 |
9 |
7 |
10 |
| Electrophoresis |
|
|
| SDS-PAGE |
• |
• |
• |
• |
– |
– |
• |
| Accurate MW estimation |
• |
• |
• |
• |
– |
– |
• |
| Multicolored |
– |
– |
– |
– |
– |
– |
– |
| Coomassie staining |
• |
• |
• |
• |
• |
• |
• |
| Fluorescent staining |
• |
• |
• |
• |
– |
• |
• |
| 2-D electrophoresis |
– |
– |
– |
– |
– |
• |
– |
| IEF |
– |
– |
– |
– |
• |
– |
– |
Plug format for use in gels with no reference well |
– |
– |
– |
– |
– |
– |
• |
| Blotting |
|
|
| Monitoring transfer efficiency |
– |
– |
– |
– |
– |
– |
– |
| Coomassie staining |
• |
• |
• |
• |
– |
• |
• |
| Fluorescent staining |
• |
• |
• |
• |
– |
• |
• |
| Fluorescent blotting* |
– |
– |
– |
– |
– |
– |
– |
| Immonodetection** |
– |
– |
– |
– |
– |
– |
• |
| Catalog Numbers |
|
|
| Single unit |
161-0317 |
161-0304 |
161-0303 |
161-0326 |
161-0310 |
161-0320 |
161-0378 |
| Value pack of 5 units |
– |
– |
– |
– |
– |
– |
– |
* These standards have fluorescent properties and can be used for fluorescent blotting applications. See bulletin 5723 for details on using Precision Plus Protein WesternC standards for fluorescent multiplexing.
Precision Plus Protein Dual Xtra standards (161-0377) are recommended for fluorescent blot analysis of proteins between 5 and 250 kD.
** Immunodetection via addition of a Precision Protein™ StrepTactin and horseradish peroxidase (HRP) or StrepTactin and alkaline phosphatase (AP) conjugate, which will bind to the internal Strep-tags on the proteins.
*** 24 unstained plugs for 2-D gels.


* These standards have fluorescent properties and can be used for fluorescent blotting applications. See bulletin 5723 for details on using Precision Plus Protein WesternC standards for fluorescent multiplexing.
Note: Value packs of 5 are available for the following Precision Plus Protein standards: All Blue (161-0393), Dual Color (161-0394), Kaleidoscope (161-0395), Dual Xtra (161-0397), WesternC (161-0398), and Unstained (161-0396)
Strep-tag technology for western blot detection is covered by U.S. Patent Number 5,506,121 and by UK Patent Number 2,272,698.
StrepTactin is covered by German patent application P 19641876.3. Bio-Rad Laboratories, Inc. is licensed by Institut für Bioanalytik GmbH to sell these products for research use only.
NOTICE TO PURCHASER: LIMITED LICENSE
*This product is sold under license from Life Technologies Corporation, Carlsbad, CA for use only by the buyer of the product. The buyer is not authorized to sell or resell this product or its components.