PrimePCR™ Assay:HMBS, Homo sapiens

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:  
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

Assay Information

Assay Design:   exonic
Chromosome Location:   11:118958440-118958555question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000539986

Gene Information

This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   HMBS
Gene Name:   hydroxymethylbilane synthase
Aliases:   PBG-D, PBGD, PORC, UPS
RefSeq:   NC_000011.9 NG_008093.1 NT_033899.8
Ensembl:   ENSG00000256269
Entrez:   3145
UniGene:   Hs.82609


Products used to generate validation data:

Real-Time PCR Instrument
Reverse Transcription Reagent
Real-Time PCR Supermix
Experimental Sample

Summary Data:

R2
y-intercept
Efficiency

Amplification Plot
Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev B Click to download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev A Click to download
Find Products & Information
Search
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)