PrimePCR™ Assay:GAPDH, Homo sapiens

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Assay Design:   exonic
Chromosome Location:   12:6643143-6643359question
Amplicon Length:   187
Splice Variants Targeted:   ENST00000229239

Gene Information

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2012]

Gene Symbol:   GAPDH
Gene Name:   glyceraldehyde-3-phosphate dehydrogenase
Aliases:   G3PD, GAPD, MGC88685
RefSeq:   NC_000012.11 NG_007073.2 NT_009759.16
Ensembl:   ENSG00000111640
Entrez:   2597
UniGene:   Hs.544577


Products used to generate validation data:

Real-Time PCR Instrument
Reverse Transcription Reagent
Real-Time PCR Supermix
Experimental Sample

Summary Data:

R2
y-intercept
Efficiency

Amplification Plot
Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev B Click to download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev A Click to download
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