Hydrophobic Interaction Chromatography Media

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Macro-Prep HIC Media
Bio-Beads SM-2 Adsorbents

Overview

Media are designed specifically for intermediate purification steps that remove host-cell contaminants from partially purified targets using strong or weak hydrophobic interactions.

Specifications

Properties of Bio-Beads SM-2

Characteristics of Macro-Prep HIC Media

Macro-Prep HIC Media

Dynamic Binding Capacity

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Macro-Prep HIC Media
Methacrylate-based beads for protein and nucleic acid purification using hydrophobic interactions. Methyl and t-butyl supports are available for purifying compounds that have weak or strong ...More »

Bio-Beads SM-2 Adsorbents
Bio-Beads SM-2 nonpolar polystyrene adsorbents are analytical-grade, neutral macroporous polymeric beads for use in hydrophobic interaction chromatography.

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	Macro-Prep t-Butyl	Macro-Prep Methyl
Functional Groups	COO^- and t-butyl	COO^- and methyl
Ionic capacity	Approximately 120 µeq/ml	Approximately 120 µeq/ml
Shipping counterion	Cl^-	Cl^-
Hydrophobicity	Mild	Mild
Median particle size (µm)	50	50
pH stability	1–10	1–10
Autoclavability (121°C, 30 min)	Yes	Yes
Shipping solution	20% ethanol	20% ethanol
Working flow-rate range	100–600 cm/hr	100–600 cm/hr
Sanitization	5 CV of 6 M guanidine-HCl 100 cm/hr or 5 CV of 1% acetic acid in 0.12 M phosphoric acid pH 1.5, 100 cm/hr	5 CV of 6 M guanidine-HCl 100 cm/hr or 5 CV of 1% acetic acid in 0.12 M phosphoric acid pH 1.5, 100 cm/hr
Storage at 4–40°C	20% ethanol or 1% acetic acid in 0.12 M phosphoric acid, pH 1.5	20% ethanol or 1% acetic acid in 0.12 M phosphoric acid, pH 1.5

Chemical nature	Polystyrene-divinyl-benzene
Polarity	Nonpolar
Average pore diameter (dry beads)	90 Å
Wet density (g/cc)	1.02
Capacity (Triton X-100 detergent) g/g	0.07

	Methyl HIC Support	t-Butyl HIC Support
Functional group	-CH₃	-C(CH₃)₃
Nominal particle size	50 µm	50 µm
Binding capacity*	>25 mg/ml	>15 mg/ml
Recommended maximum linear flow rate	3,000 cm/hr	3,000 cm/hr
pH stability	1–12	1–12
Regeneration	70% ethanol	70% ethanol
Autoclavability (121°C, 30 min)	Yes	Yes
Storage	20% ethanol	20% ethanol

		Retention Times (min)
Peak	Protein	Methyl	t-Butyl
1	Cytochrome c	7.3	10.41
2	Ovalbumin	32.25	40.22
3	Α-Anylase	62.81	71.5
4	Ferritin	81.49	90.9

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Hydrophobic Interaction Chromatography Media

Overview

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Hydrophobic Interaction Chromatography Media

Overview

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