Computer-assisted image analysis is an indispensable tool for the evaluation of complex 2-D gels. Image analysis software obtains quantitative and qualitative information about the proteins in a sample and stores the information in files, which may also contain additional annotations. PDQuest™ 2-D analysis software is a tool for analysis of digitized gel images that integrates with Bio-Rad's image acquisition instruments.
Spot comparison with PDQuest software. PDQuest software can be used to compare two or more gel images, revealing changes in protein expression in response to different treatments, growth conditions or disease states.
The wizard driven software uses the following work flow:
PDQuest can also be used to control the ExQuest™ spot cutter for automated spot excision.
After separation by 1-D gel electrophoresis, you can utilize a number of methods for protein imaging and analysis involving staining techniques or stain-free technology in combination with specialized imaging devices and accompanying software.