Ready Gel^® Precast Gels

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Overview

Mini format Ready Gel precast gels are for use with the Mini-PROTEAN Tetra and Mini-PROTEAN 3 Dodeca cells. The individually packaged gels are available in six formulations.

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Ready Gel Tris-HCI Precast Gels
Bio-Rad Tris-HCl gels are made without SDS. SDS is added to the sample and running buffers to create standard SDS denaturing electrophoresis conditions.

Ready Gel IEF Precast Gels
Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel.

Ready Gel Zymogram Precast Gels
Zymogram gels contain gelatin or casein, which are substrates for proteases. Samples with proteolytic activity can be visualized as clear bands against a blue background after Coomassie R-250 staining.

Ready Gel TBE-Urea Precast Gels
TBE-urea gels maintain denaturing conditions for analysis of single-stranded DNA and RNA. Nucleic acids between 60 and 200 bases are resolved as sharp, distinct bands.

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Composition of Ready Gels
	Resolving Gel (%T)	Stacking Gel (%T)	Crosslinks	Optimized Separation Range	Application
Tris-HCl Gels	5%	4%	2.60%	75–200 kD	SDS-PAGE Native PAGE
	7.50%	4%	2.60%	65–200 kD	SDS-PAGE Native PAGE
	10%	4%	2.60%	21–200 kD	SDS-PAGE Native PAGE
	12%	4%	2.60%	14–100 kD	SDS-PAGE Native PAGE
	15%	4%	2.60%	6.5–200 kD	SDS-PAGE Native PAGE
	18%	4%	2.60%	6.5–100 kD	SDS-PAGE Native PAGE
	4–15%	None	2.60%	40–200 kD	SDS-PAGE Native PAGE
	4–20%	None	2.60%	6.5–200 kD	SDS-PAGE Native PAGE
	8–16%	4%	2.60%	6.5–200 kD	SDS-PAGE Native PAGE
	10–20%	4%	2.60%	6.5–100 kD	SDS-PAGE Native PAGE
Tris-Borate-EDTA	5%	None	3.30%	200–1,750 base pairs	Nucleic acid electrophoresis
	5%	None	3.30%	50–1,750 base pairs	Nucleic acid electrophoresis

Precast Gel System Selection Guide
	Polyacrylamide			Agarose
	Ready Gel^® System	Criterion™ System	PROTEAN^® II Ready Gel System	ReadyAgarose™ System
Gel dimensions (W x L)	8.6 x 6.8 cm	13.3 x 8.7 cm	16 x 16 cm 18.3 x 19.3 cm	6.6 x 10 cm 15 x 10 cm
Gel cassette dimensions (W x L)	10 x 8 cm	15 x 10.6 cm	20 x 18.3 cm 20 x 22.3 cm	7.1 x 10 cm 15.6 x 10 cm
Precast gel type	Ready Gel	Criterion and Criterion XT	PROTEAN II	ReadyAgarose mini ReadyAgarose wide mini ReadyAgarose 96 Plus
Electrophoresis cells	Mini-PROTEAN^® Tetra Mini-PROTEAN^® 3 Dodeca™	Criterion Criterion Dodeca	PROTEAN II xi/XL PROTEAN II xi/XL multi-cells	Mini-Sub^® cell GT Mini ReadySub-Cell™ GT Wide Mini-Sub cell Wide mini ReadySub-Cell GT
Blotting cell	Mini Trans-Blot^® Trans-Blot^® SD	Criterion Trans-Blot SD	Trans-Blot Trans-Blot Plus Trans-Blot SD	Model 785 Trans-Blot SD

Gel Size (W x L)	Precast Gel Types (Polyacrylamide)	Comb Types	Electrophoresis Cells	Blotting Cells
8.6 x 6.8 cm	Tris-HCl, Tri-Tricine, IEF, zymogram, TBE, TBE-urea	10-well (30 µl), 15-well (15 µl), prep well (450 µl), 10-well (50 µl), 12-well (20 µl), 9-well (30 µl), IPG comb (7 cm IPG strip)	Mini-PROTEAN Tetra cell or Mini-PROTEAN^® 3 Dodeca™ cell	Mini Trans-Blot^® cell

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Ready Gel^® Precast Gels

Overview

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Ready Gel® Precast Gels

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Ready Gel^® Precast Gels

Category Products