Semi-Dry and Rapid Blotting Systems

Higher sensitivity and better transfer efficiency is seen with the Trans-Blot^® Turbo™ system in comparison to other blotting techniques. This data set demonstrates the successful transfer of the 1.25 ng protein band only with the Trans-Blot Turbo system. Serial dilutions of transferrin were separated on a Criterion™ TGX™ 4–20% gel and transferred using four different blotting techniques. All images are adjusted to the same settings.

2x Protein Transfer

Quantitation performed on equivalent 2-D gels transferred with the Trans-Blot^® Turbo™ and the iBlot systems demonstrated twice the number of proteins transferred and detected with the Trans-Blot Turbo system. Rat liver extract was focused on Bio-Rad's ReadyStrip™ 11 cm pH 5–8 IPG strips and separated on AnykD™ Criterion™ TGX™ gels. Duplicate gels were transferred with the Trans-Blot Turbo and iBlot systems, both using manufacturers’ recommended 7 min protocol. The nitrocellulose membranes were subsequently stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system.

Turbo Transfers with Trans-Blot^® Turbo™ Transfer Packs

• 3-minute protocol — a single Mini-PROTEAN^® TGX™ gel (for proteins with MW 5–150 kD) can be transferred in as little as 3 min
• 7-minute protocol — up to 4 mini or 2 midi gels with mixed-molecular weight proteins (MW 5–150 kD) can be efficiently transferred in 7 min
• 10-minute protocol — up to 4 mini or 2 midi gels with high-molecular weight proteins (MW 25–300+ kD) can be efficiently transferred in 10 min

E. coli lysate (6 µg) was diluted two-fold. Samples were separated with Mini-PROTEAN TGX gels, transferred with the Trans-Blot Turbo system, stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system. Standards in lane 1 are Precision Plus Protein™ Unstained standards, with a top band of 250 kD MW.

Bio-Rad Trans-Blot SD Semi-Dry Blotting Apparatus

Trans-Blot SD Semi-Dry Assembly Guide