RNA Isolation Selection Guide
| |
Aurum Total RNA Kits |
PureZOL RNA Isolation Reagent |
| Mini |
Fatty and Fibrous Tissue |
96 |
| Format |
Mini column Filtration (vacuum or spin) |
Mini column Filtration (vacuum or spin) |
96-well plate Filtration (vacuum or spin) |
— |
| Maximum starting amounts |
|
|
|
|
| Cultured cells |
2 x 106 |
— |
1 x 106 |
1 x 107 |
| Bacterial cells |
2.4 x 109 |
— |
8 x 108 |
2.4 x 109 |
| Yeast cells |
3 x 107 |
— |
2 x 107 |
3 x 107 |
| Animal tissue — hard |
20 mg |
100 mg |
— |
100 mg |
| Animal tissue — soft to moderately hard |
40 mg |
100 mg |
— |
100 mg |
| Plant tissue |
40 mg |
100 mg |
— |
100 mg |
| Isolation method |
Silica membrane |
Lysis with PureZOL reagent, purification on silica membrane |
Silica membrane |
Organic extraction |
| Number of preps |
10* or 50 mini preps |
10* or 50 mini preps |
2 x 96-well plate |
50 or 100 preps (1 ml/prep) |
| Number of washes |
3 |
3 |
3 |
— |
| DNase I included** |
Yes |
Yes |
Yes |
No |
| DNase I digest time |
15 min (animal tissue, 25 min) |
15 min |
10 min |
— |
| Total preparation time |
<50min (1.5 hr with DNase I) |
<50min (1.5 hr with DNase I) |
<60 min |
<60 min |
| Binding capacity |
>100 µg |
>100 µg |
>40 µg |
— |
| Elution volume |
2 x 40 µl |
2 x 40 µl |
80 µl |
30–100 µl |
| Yield*** |
28 µg/2 x 106 NIH 3T3 cells |
96 µg/100 mg brain |
10 µg/1 x 106 NIH 3T3 cells |
1.15 µg/mg brain |
| |
38 µg/2 x 106 HeLa cells |
58 µg/100 mg breast |
17 µg/1 x 106 HeLa cells |
1.37 µg/mg fat |
| |
20 µg/40 mg brain |
85 µg/100 mg heart |
11 µg/2 x 107 S. cerevisiae cells |
1.2 µg/mg heart |
| |
52 µg/20 mg thymus |
63 µg/100 mg skin |
5 µg/8 x 108 E. coli cells |
0.66 µg/mg cartilage |
| |
132 µg/40 mg spleen |
54 µg/100 mg cartilage |
5 µg/8 x 108 B. cereus cells |
1.2 µg/mg skin |
| |
36 µg/40 mg lung |
|
|
1.0 µg/mg potato |
| |
32 µg/40 mg potato |
|
|
1.37 µg/1 x 105 293H cells |
| |
28 µg/3 x 107 S. cerevisiae cells |
|
|
0.1 µg/mg Arabidopsis |
| |
54 µg/2.4 x 109 E. coli cells |
|
|
0.38 µg/mg A. niger |
| |
|
|
|
0.63 µg/2.4 x 107 E. coli cells |
| |
|
|
|
0.31 µg/3 x 105 S. cerevisiae cells |
| Purity |
A260/A280 of 1.9–2.1 |
A260/A280 of 1.9–2.1 |
A260/A280 of 1.9–2.1 |
A260/A280 of 1.9–2.1 |
* Trial sizes of the Aurum total RNA mini kit and Aurum total RNA fatty and fibrous tissue kit are available.
** Removal not required.
*** Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.
