2-D Electrophoresis Workflow

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2-D Electrophoresis Workflow

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2-D Electrophoresis Workflow How-To Guide

2-D Electrophoresis Workflow How-To Guide

Guides You Through 2-D Electrophoresis From Start to Finish

This manual provides in-depth information about:

  • Experimental methods and tools
  • Standard protocols
  • Ideas for developing customized protocols
  • Tips and tricks to improve results and reproducibility

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2-D Doctor

Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor™

The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.

Learn more

PROTEAN® i12™ IEF System Tour

12 Lanes. Individual Control. Total Confidence.

 

Simultaneously run 12 lanes, each with different samples, pH gradients, and protocols

  • Optimize experiments in fewer runs
  • Run multiple samples at once
  • Get more reproducible results

Learn how to:

  • Assemble trays, electrodes, and
    load samples
  • Use the easy touch-screen control to program and operate the system
  • Use the free web-based application for data analysis and protocol design

Launch the System Tour

2-D Video Tutorial

Bio-Rad's 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D results.

5 Steps

  1. Sample Preparation — sample preparation is the first step toward optimizing your results. Bio-Rad offers easy-to-use kits: ReadyPrep™ 2-D starter kit, ReadyPrep 2-D cleanup kit, and ProteoMiner™ protein enrichment kit for getting started and for cleanup and enrichment of low-abundance proteins.
  2. First Dimension Electrophoresis — the first dimension of 2-D electrophoresis is separation based on a protein's isoelectric point. Bio-Rad's IPG strips offer many pH gradient options and strict quality control. The award-winning PROTEAN® i12™ IEF system gives you the flexibility to run multiple pH gradients, protocols, and samples simultaneously.
  3. Second Dimension Electrophoresis — the second dimension of 2-D electrophoresis is separation based on molecular weight. Bio-Rad's Criterion™ system, Criterion precast gels, Criterion™ Dodeca™ cell, and Precision Plus Protein™ standard plugs provide the confidence of a leakproof precast gel and the ability to run up to 12 gels at once.
  4. Staining, Imaging, and Analysis — staining, imaging, and analysis allow you to document and evaluate your 2-D results. With Bio-Rad's sensitive and hassle-free Oriole™ gel stain and versatile ChemiDoc™ MP imager, you can obtain high-quality images. Analyze those images with PDQuest™ software, a highly powerful tool for 2-D evaluation.
  5. Spot Cutting — to analyze protein spots from your 2-D gel by mass spectrometry, they must be cut from the gel. Automate this process with the EXQuest™ spot cutter. Then import your cut list from the software and the instrument will do the rest.

     
    2-D Workflow

Applications and Technical Resources

The 2-D electrophoresis applications and technology page is a virtual encyclopedia of 2-D electrophoresis. You'll find extensive information, videos, and troubleshooting guides to ensure you get the best 2-D results possible.

On the 2-D Electrophoresis applications and technology page you can:

PROTEAN i12 IEF system
PROTEAN® i12™ IEF system

164-6000
Isoelectric focusing system, includes basic unit, electrode assemblies, focusing and rehydration/equilibration trays, forceps, electrode wicks, mineral oil, cleaning accessories, IPG strips and sample/rehydration buffer, USB flash drives, styluses

Precio de lista:   Consultar
Cantidad:   cotización
lista de preferencias  
ChemiDoc MP System
ChemiDoc™ MP System

170-8280
Gel imaging system, includes darkroom, UV transilluminator, epi-white illumination, camera, power supply, cables, Image Lab™ software compatible with Windows or Mac OS

Precio de lista:   Consultar
Cantidad:   cotización
lista de preferencias  
Criterion™ Dodeca™ Cell and PowerPac™ Universal Power Supply

165-4139
Vertical midi-format electrophoresis cell (#165-4130), 12 gel capacity, includes buffer tank with cooling coil, lid with power cables, universal power supply (#164-5070)

Precio de lista:   Consultar
Cantidad:   cotización
lista de preferencias  
EXQuest Spot Cutter

165-7200
Gel excision instrument, includes enclosure, imaging system, fluidics system, robotics, sensors, cutting head, gel tray, microplate track, wash station

Precio de lista:   Consultar
Cantidad:   cotización
lista de preferencias  
PDQuest Advanced 2-D Analysis Software

170-9630
1-user license, 2-D analysis software, provides advanced functionality

Precio de lista:   Consultar
Cantidad:   cotización
lista de preferencias  
Number Descripción Opciones
6478 2-D Electrophoresis Workflow Brochure, Rev A Click to download
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide, Rev A Click to download
2651 2-D Electrophoresis for Proteomics: A Methods and Product Manual, Rev F Click to download
5635 ProteoMiner Protein Enrichment Technology Bulletin, Rev B Click to download [ Add to Cart (Free) ]
10010636 Instruction Manual, ProteoMiner Protein Enrichment Kits, Rev D Click to download
5916 Enrichment of Medium- and Low-abundance Proteins in Sample Types Using ProteoMiner Technology, Rev A. Click to download
4110143 Instruction Manual, ReadyPrep 2-D Cleanup Kit, Rev A Click to download [ Add to Cart (Free) ]
4110009 Instruction Manual, ReadyPrep 2-D Starter Kit, Rev A Click to download
2642 ReadyPrep 2-D Starter Kit Product Information Sheet Click to download
6097 PROTEAN i12 IEF System Brochure, Rev A Click to download [ Add to Cart (Free) ]
6138 PROTEAN i12 IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions, Rev A Click to download
4006166 Instruction Manual, ReadyStrip IPG Strip, Rev H Click to download
2442 ReadyStrip IPG Strip Product Information Sheet, Rev F Click to download
4006197 Instruction Manual, Criterion Dodeca Cell, Rev A Click to download
6040 Electrophoresis Guide, Interactive PDF, Rev B Click to download
6007 Criterion TGX Precast Gels Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
4110001 Instruction Manual, Criterion Gel Application Guide, Rev F Click to download
5991 Oriole Fluorescent Gel Stain: A Novel Fluorescent Stain for a Simpler 2-D PAGE to MALDI Workflow, Rev A Click to download
10017295 Instruction Manual, Oriole Fluorescent Gel Stain, Rev B Click to download
5900 Oriole Fluorescent Gel Stain PIS, Rev A Click to download
6133 ChemiDoc MP Brochure, Rev C Click to download [ Add to Cart (Free) ]
3121 PDQuest 2-D Analysis Software Brochure, Rev C Click to download
3194 EXQuest Spot Cutter Brochure, Rev A Click to download [ Add to Cart (Free) ]
10001531 Instruction Manual, EXQuest Spot Cutter, Rev A Click to download
6371 Electrophoresis Power Supplies Brochure, Rev C Click to download
6393 Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins, Rev A Click to download
6405 Effectiveness of 2-D Electrophoresis over 1-D Electrophoresis Followed by Western Blotting for Evaluating Strategies Used to Generate Host Cell Protein Antibodies, Rev A Click to download