Pierceable Foil Heat Seal

Imprimir
Pierceable Foil Heat Seal

Visión general

Suggested Heat Sealing Parameters

 

        Optically Clear Heat Seal Permanent Clear Heat Seal Pierceable Foil Heat Seal Peelable Foil Heat Seal
Product Catalog Number Number of Wells Well Color Temp, °C Time, sec Temp, °C Time, sec Temp, °C Time, sec Temp, °C Time, sec
Hard-Shell® 384-well standard PCR plates HSP-3xxx 384 Clear

White
175

167
3

3
175

167
3

3
170

170
3

3
185

185
4

3
Hard-Shell 384-well 480 PCR plates HSR-4xxx 384 Clear

White
175

167
3

3
175

167
3

3
170

170
3

3
185

185
4

3
Hard-Shell low-profile 96-well skirted PCR plates HSP-9xxx 96 Clear

White
185

180
3

3
180

180
3

3
175

175
3

3
180

185
3

5
Hard-Shell high-profile 96-well semi-skirted PCR plates HSS-9xxx 96 Clear

White
170

185
3

3
165

180
3

3
170

175
3

3
185

185
3

3
Multiplate™ low-profile 96-well unskirted PCR plates MLL-9xxx 96 Clear

White
180

180
3

3
180

160
3

3
175

175
3

3
185

185
5

3
Multiplate high-profile 96-well unskirted PCR plates MLP-9xxx 96 Clear

White
175

170
3

3
150

165
3

3
175

175
3

3
185

185
3

3
iQ™ 96-well PCR plates 223-9441 96 Clear 180 3 180 3 185 3 185 5

 

Heat Sealing Films and Foils
  Optically Clear
Heat Seal
Permanent Clear
Heat Seal
Pierceable Foil
Heat Seal
Peelable Foil
Heat Seal
Catalog number 181-4030 181-4035 181-4040 181-4045
Applications – Real-time PCR
– PCR
– Water bath cycling
– PCR
– QX100™ Droplet Digital™
  PCR system
– PCR
– Sample storage
– PCR
Maximum temperature 110°C 110°C 110°C 110°C
Minimum temperature –80°C –80°C –20°C –200°C
Peelable seal +   + +
Pierceable seal     +  
DNase and RNase free + + + +
Sealing compatibility PP, COC, PE,* PS PP PP, PS* PP, COC*
COC, cyclic olefin copolymer; PE, polyethylene; PP, polypropylene; PS, polystyrene.
* Limited and variable compatibility based on plate composition.

PX1™ Heat Sealing Performance

 

Integrating the PX1 PCR Plate Sealer into the Thermal Cycling Workflow Helps Generate Highly Reproducible and Uniform PCR Data

Real-time PCR experiments using the PX1 PCR plate sealer in the workflow demonstrate highly uniform amplification curves. A, a SYBR® Green uniformity assay examined amplification uniformity across an entire 96-well plate sealed using the PX1 PCR plate sealer. The extremely low quantification cycle (Cq) standard deviation of all 96 wells (0.09 cycles) demonstrates exceptional experimental uniformity. B, the SYBR® Green uniformity melt curve analysis demonstrates a single, consistent melt peak across all wells, which indicates product specificity is maintained in plates sealed with the PX1 PCR plate sealer. RFU, relative fluorescence units.

The optically clear heat seal prevents sample evaporation that can lead to large Cq standard deviations. Five-color multiplex linearity test demonstrates highly reliable qPCR results. A–E, fluorescence data from a series of tenfold dilutions of plasmid DNA (108–102 copies) amplified using reporter dyes to monitor five targets. The standard curves of five multiplexed genes have an R2 >0.990. F, standard curves generated from data in A–E. Reaction efficiencies are between 90 and 105%. Cq, quantification cycle; RFU, relative fluorescence units.

PX1™ PCR Plate Sealer FAQs

Which heat seal is best for my PCR application?
Use the Heat Sealing Films and Foils table to determine the seal that best suits your experimental needs.

 

How do I determine the best sealing time and temperature?
Optimal sealing parameters vary according to the heat films and microplates used. We recommend sealing an empty plate using the guidelines found in Suggested Heat Sealing Parameters. After sealing, examine the entire plate for effective sealing and optimize sealing conditions if necessary.

 

Some of my wells are not fully sealed. What should I do?
Sealing temperature and/or sealing time may be increased to help improve sealing performance. Use an empty plate to optimize seal conditions. See the instruction manual for additional details about seal optimization.

 

When should I use the sealing frame?
The sealing frame helps secure the heat seal in place and prevents the film from moving during sealing. The sealing frame must be used for the permanent clear heat seal and the peelable foil heat seal.

 

What happens if I accidently place a seal upside down when sealing?
The PX1 heated platen was designed with an easy to clean, nonstick surface. See the PX1™ PCR Plate Sealer Instruction Manual for directions on cleaning the heated platen. Ensure the PX1 sealer cools for at least 1 hour before cleaning.

 

Is it safe to leave the heat sealer on when unattended?
Yes. The PX1 PCR plate sealer was designed for reliability and safety. The instrument has an adjustable auto-off feature that automatically shuts the heater off when not in use for a designated amount of time. Simply touch the screen and the instrument will automatically resume heating.

 

Can I reseal the same plate twice?
A plate sealed with a peelable film may be resealed after peeling an existing film. However, resealing a plate for subsequent use in real-time PCR is not recommended. A plate sealed with a pierceable foil heat seal may also be resealed by sealing another pierceable foil heat seal directly on top. Note: only a single seal can be used in the QX100™ Droplet Digital™ PCR system workflow. Sealing parameters may need to be adjusted.

 

The current platen temperature is higher than the required sealing temperature. How can I reduce the time needed to reach sealing temperature?
The heated platen cools passively. Opening the PX1 PCR plate sealer drawer can help accelerate the cooling process.

 

Can I use traditional adhesive or pressure-sensitive seals with the PX1 sealer?
No. Our heat sealing films feature a special hot melt adhesive that effectively seals microplates when used with the PX1 sealer. The temperature and pressure delivered by the PX1 will not successfully seal a microplate when traditional adhesive and pressure-sensitive seals are used.

Amplification Reagents and Plastics Brochure
The Amplification Reagents and Plastics Brochure includes information about

 

  • Products for gene expression analysis


    RNA Isolation


    Reverse Transcription Reagents


    Real-Time qPCR Reagents


    PCR Plastic Consumables

  • Product performance data

  • Quick and easy product selection tables

  • Ordering information

 

Download your free copy of the Amplification Reagents and Plastics Brochure.

The pierceable foil heat seal provides a secure sealing option for standard and Droplet Digital PCR applications. This easily pierceable aluminum film enables sample retrieval from select wells. Microplates with pierced seals can be resealed with another pierceable foil heat seal on top.

Key Features and Benefits

  • Compatible with Droplet Digital PCR — independently validated in the QX100™ Droplet Digital™ PCR system workflow
  • Fully validated — minimal sample evaporation when used with the PX1™ PCR plate sealer
  • Solvent resistant — helps prevent seal breakdown when reagents containing high DMSO concentrations are used
  • User friendly — colored stripe clearly identifies sealing surface

Additional Details

Bio-Rad's Heat Sealing Films and Foils

Bio-Rad offers several heat sealing films and foils, each of which is optimal for specific PCR applications.

Heat Seal Application
Optically clear heat seal Best suited for thermal cycling and real-time PCR applications when a clear, peelable seal is desired
Permanent clear heat seal Provides the strongest heat sealing option and is ideal for thermal cycling experiments where seals do not need to be removed
Pierceable foil heat seal Ideal for thermal cycling and workflows requiring a pierceable seal, such as Droplet Digital PCR applications
Peelable foil heat seal Best for thermal cycling and sample storage
Find out more about the specific features and specifications for each of the heat sealing films and foils.

Pierceable Foil Heat Seal

181-4040
Pkg of 100, foil seals for PCR and QX200™/QX100™ Droplet Digital™ PCR System applications, for use with PX1™ PCR Plate Sealer

Precio de lista:   Consultar
Cantidad:   cotización
lista de preferencias  
Number Descripción Opciones
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
10025821 Pierceable Foil Heat Seal Product Insert, Rev A Click to download
10023997 Instruction Manual, PX1 PCR Plate Sealer, Rev A Click to download
6257 PX1 PCR Plate Sealer and Heat Sealing Consumables Brochure, Rev A Click to download