Bio-Plex Pro Human Cytokine 27-plex Assay #M500KCAF0Y

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Overview

1 x 96-well, includes coupled magnetic capture beads, premixed detection antibodies, standards, quality control, detection antibody diluent HB, standard diluent HB, sample diluent HB, assay buffer, 10x wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions for the detection of 27 human cytokine biomarkers.

Safety Data Sheet

Safety Data Sheet

Description

Description

The Bio-Plex Pro Human Cytokine Screening Panel enables you to interrogate 27 cytokine and chemokine cell signaling molecules in complex extracellular events and signaling pathways involved in heart disease, Alzheimer's disease, autoimmunity and allergy, infectious diseases, inflammation, cancer, and neurodegeneration. This high-performance, broad screening panel consists of a biologically-relevant collection of adaptive immunity cytokines, pro-inflammatory cytokines, and anti-inflammatory cytokines all in a single well. Achieve accurate, actionable results with the Bio-Plex Pro™ Human Cytokine Screening Panel, 27-plex. This panel includes:

    • FGF basic
    • Eotaxin
    • G-CSF
    • GM-CSF
    • IFN-γ
    • IL-1β
    • IL-1ra
    • IL-2
    • IL-4
    • IL-5
    • IL-6
    • IL-7
    • IL-8
    • IL-9
    • IL-10
    • IL-12 (p70)
    • IL-13
    • IL-15
    • IL-17A
    • IP-10
    • MCP-1 (MCAF)
    • MIP-1α
    • MIP-1β
    • PDGF-BB
    • RANTES
    • TNF-α
    • VEGF

Based on 6.5 µm magnetic beads, it is compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic beads for simplified plate processing
  • All-in-one, space-saving complete kit
  • Premixed standards for generation of curves over a broad dynamic range for each target

Other Assay Formats Available

  • Custom premixed assays: available as Bio-Plex x-Plex Assays
  • Custom assays (mixed at the bench): available as Bio-Plex Express Assays
  • Singleplex sets (each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires a reagent kit for magnetic separation (171304090M) and a vial of standards (171D50001) for a complete assay)

For multiplexing capabilities with other cytokines, refer to Bulletin 5927. For multiplexing capabilities with diabetes assays, refer to Bulletin 5975.

Instrument Compatibility

Related Products

Bio-Plex Pro Human Inflammation Assays
For the first time, the key biomarkers of inflammation from the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs can be measured using a single multiplex kit. Bio-Plex Pro Human Inflammation Assays are available in larger 24-plex and 37-plex screening panels and a pathway-focused Treg 12-plex panel, along with singleplex and custom-configured assays.

Specifications

Specifications

Species
Human
Immunoassay for
Cytokines
Format
Premixed panel
Custom assay options
x-Plex™ assays
Compatible with
Bio-Plex® 200 system, Bio-Plex 3D system, Bio-Plex Pro™ wash station, and Bio-Plex Pro II wash station

Recommended SKUs

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