Hard-Shell Full-Height 96-Well Semi-Skirted PCR Plates #HSS9665


Pkg of 25, black shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

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Hard-Shell 96-well semi-skirted PCR plates are specifically designed to withstand the stresses of thermal cycling and robotic handling. The patented* two-component design provides superior stability and flatness, allowing precise positioning for automation. The full-height (20.75 mm) wells fit most thermal cyclers, real-time PCR detection systems, and DNA sequencers.

Key Features and Benefits

  • Reaction volumes of 5–125 µl (350 µl maximum)
  • Extremely uniform wells reduce well-to-well variability in optical assays, such as those used in real-time PCR
  • Warp-free half-height skirt for improved robotic handling and labeling surface
  • Black lettering for easy well identification
  • Process sampling and testing to ensure plates are free of DNase, RNase, and human DNA


  • White-well option for increased fluorescent signal strength
  • Color-coded skirt option with clear wells
  • Low-cost, user-readable bar code option for convenient database tracking

Plate Dimensions

Microplate Dimensions Hard-Shell® High-Profile 96-Well Semi-Skirted PCR Plates (HSS-9xxx)
Length at top plane 127.76 mm
Width at top plane 85.47 mm
Height overall 20.75 mm
Well depth 19.85 mm
Well diameter at opening 5.50 mm
Well diameter at bottom of conical section 2.69 mm
Well volume 350 μl
Well spacing 9.00 mm
Well angle 17.5°
Well offset
  Left edge to well A1 14.37 mm
  Top edge to well A1 11.23 mm
  Left edge to H12 113.37 mm
  Top edge to H12 74.23 mm

* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.