Hard-Shell® 96-Well 480 PCR Plates #HSR9905

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Pkg of 25, clear shell/white well PCR plate for Roche LightCycler 480 Real-Time PCR System, barcoded, rigid 2-component design

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Well/tube format 96-well
Well/tube profile Low
Skirt format Engineered for optimal fit in Roche LightCycler 480
Compatible PCR instrument Roche LightCycler 480
Well/tube color White

Hard-Shell 96-well 480 PCR plates are optimized to work on the Roche LightCycler 480 real-time PCR system. Hard-Shell plates are specifically designed to withstand the stresses of heat sealing, thermal cycling, and robotic handling. The patented* two-component microplate design provides superior stability and flatness, allowing precise positioning for automation. The low-profile wells are optimized for low-volume reactions and fast PCR.

Key Features and Benefits

  • Polycarbonate shell — prevents plate warping when thermal cycling in the Roche LightCycler 480
  • Extremely uniform wells — reduce well-to-well variability in optical assays performed in real-time PCR
  • Black lettering — facilitates well identification
  • Rigid shell — ideal for heat sealing methods with instruments such as the PX1™ PCR plate sealer

Plate Dimensions

Hard-Shell 96-Well 480 PCR Plate Dimensions

Not drawn to scale.
All dimensions in mm, unless otherwise noted.

Microplate Dimensions Hard-Shell® 96-Well 480 PCR Plates (HSR-99xx)
Length at base plane 127.76 mm
Width at base plane 85.48 mm
Height overall 15.51 mm
Well depth 14.41 mm
Well diameter at opening 5.46 mm
Well diameter at bottom of conical section 3.00 mm
Well volume 200 μl
Well spacing 9.00 mm
Well angle 17.0°
Well offset  
    Left edge to well A1 14.38 mm
    Top edge to well A1 11.24 mm
Center of A1 to center of A12 99.00 mm
Center of A1 to center of H1 63.00 mm

Additional Details

  • White-well option for increased fluorescent signal strength
  • Bar-coded on row A side for convenient database tracking
  • Footprint and well spacing match ANSI/SBS standard dimensions
  • Reaction volumes of 5–125 µl (200 µl maximum)
  • Process sampling and testing to ensure that plates are free of DNase, RNase, and human DNA

Compatible PCR Instrument

  • Roche LightCycler 480 real-time PCR system

* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.