12% Criterion™ TGX Stain-Free™ Protein Gel, 12+2 well, 45 µl #5678043

12% Criterion™ TGX Stain-Free™ Protein Gel, 12+2 well, 45 µl

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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Gel percentage 12%
Well Number (Vol) 12+2 (45 µl)

Use this midi Criterion TGX Protein Gel for separation of polypeptides from ~6–200 kD. The novel formulation of TGX, Tris-Glycine eXtended, Stain-Free Gels delivers a long shelf life and allows rapid fluorescent detection of proteins with Bio-Rad’s stain-free enabled imaging systems. TGX Stain-Free Gels can be used for either standard SDS denaturing protein separations or native electrophoresis.

Bio-Rad offers a wide range of Criterion TGX Stain-Free Precast Protein Gels, including other 12+2-well gels in both single percentages and gradients.

Run up to four gels using the Criterion Cells, or for higher throughput the Criterion Dodeca Cell can run up to 12 gels or IPG strips.

Features and Benefits

  • Use SDS-containing running and sample buffers for denaturing electrophoresis
  • In the presence of SDS TGX gels have Laemmli-like separations
  • Run time as short as 20 min
  • Without SDS proteins retain native conformation and activity
  • Two wells for markers or other references
  • 12 month shelf life

Criterion TGX Stain-Free Gels contain trihalo compounds, which in the presence of UV-light react with tryptophan residues producing fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting, and may be visualized in gels or multiple times on PVDF membranes.

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Supporting Documents

Criterion Gel Application Guide, Instruction Manual