RC DC™ Protein Assay Kit I #5000121

Print

Colorimetric assay kit, includes RC Reagents Package, DC™ Protein Assay Reagents Package, bovine γ-globulin standard; 450 standard assays

5000121 [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [54.221.17.234, 10.232.19.41] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/sku/5000121-rc-dc-protein-assay-kit-i] [x-amzn-trace-id] = [Root=1-5a8dc643-7f9ff2f81b6b73ba30b13521] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=5000121] [host] = [10.232.1.21:1776] [x-request-uri] = [/en-us/sku/5000121-rc-dc-protein-assay-kit-i] [connection] = [Keep-Alive] [accept-encoding] = [x-gzip, gzip, deflate] [user-agent] = [CCBot/2.0 (http://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 5000121-rc-dc-protein-assay-kit-i en US en-us <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="development"> <Deployment Id="StandardMetadata.jddgmm4tjddgmm4u" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jddgmm4tjddgmm4u"> <Name>StandardMetadata</Name> <TargetEnviroment>development</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="10" DayOfWeek="6" Year="2018" Hour="5" Minute="49" Second="4" ZoneOffsetMillis="0" Time="1518241744540">Sat Feb 10 05:49:04 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="10" DayOfWeek="6" Year="2018" Hour="5" Minute="49" Second="4" ZoneOffsetMillis="0" Time="1518241744540">Sat Feb 10 05:49:04 UTC 2018</LastExecutionTime> </Node> <Node Name="PROD"> <Deployment Id="StandardMetadata.jddgmm4zjddgmm50" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jddgmm4zjddgmm50"> <Name>StandardMetadata</Name> <TargetEnviroment>PROD</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="10" DayOfWeek="6" Year="2018" Hour="5" Minute="52" Second="57" ZoneOffsetMillis="0" Time="1518241977238">Sat Feb 10 05:52:57 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="10" DayOfWeek="6" Year="2018" Hour="5" Minute="52" Second="57" ZoneOffsetMillis="0" Time="1518241977238">Sat Feb 10 05:52:57 UTC 2018</LastExecutionTime> </Node> </Deployments> 07/30/13 03:25 AM en_US jddgmm0t 2017-11-14 06:29:46 true true internet/generic LSE N en 07/30/20 05:00 PM 1375179935592 1518242136325 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 06:29:46 edu-price-overlay page_EDUPriceOverlay 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the high school or college level, visit our <a href="http://www.bio-rad.com/en-us/education/education-discount-policy">Education Discount Policy</a> page to establish an education account number.</p> </div> /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE N 0 5000121 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jbza3kn3jbza3kn4" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jbza3kn3jbza3kn4"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="4" DayOfWeek="4" Year="2018" Hour="23" Minute="44" Second="56" ZoneOffsetMillis="0" Time="1515109496045">Thu Jan 04 23:44:56 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette/500-0121_0921120601900.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="4" DayOfWeek="4" Year="2018" Hour="23" Minute="44" Second="56" ZoneOffsetMillis="0" Time="1515109496045">Thu Jan 04 23:44:56 UTC 2018</LastExecutionTime> </Node> <Node Name="PRODWEST"> <Deployment Id="StandardMetadata.jbza3ks6jbza3ks7" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jbza3ks6jbza3ks7"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODWEST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="5" DayOfWeek="5" Year="2018" Hour="22" Minute="4" Second="58" ZoneOffsetMillis="0" Time="1515189898804">Fri Jan 05 22:04:58 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette/500-0121_0921120601900.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="5" DayOfWeek="5" Year="2018" Hour="22" Minute="4" Second="58" ZoneOffsetMillis="0" Time="1515189898804">Fri Jan 05 22:04:58 UTC 2018</LastExecutionTime> </Node> <Node Name="PRODEAST"> <Deployment Id="StandardMetadata.jbza3ks6jbza3ksd" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jbza3ks6jbza3ksd"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODEAST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="5" DayOfWeek="5" Year="2018" Hour="22" Minute="35" Second="11" ZoneOffsetMillis="0" Time="1515191711669">Fri Jan 05 22:35:11 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette/500-0121_0921120601900.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="5" DayOfWeek="5" Year="2018" Hour="22" Minute="35" Second="11" ZoneOffsetMillis="0" Time="1515191711669">Fri Jan 05 22:35:11 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jbza3kdd 2017-11-14 07:09:01 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:54:32 5000121-rc-dc-protein-assay-kit-i 5000121 <em>RC DC</em> Protein Assay Kit I <em>RC DC</em>&trade; Protein Assay Kit I Colorimetric assay kit, includes <em>RC</em> Reagents Package, <em>DC</em>&trade; Protein Assay Reagents Package, bovine &gamma;-globulin standard; 450 standard assays Colorimetric assay kit, includes <em>RC</em> reagents package, <em>DC</em> protein assay reagents package, bovine &#947;-globulin standard; 450 standard assays Colorimetric assay kit, includes RC Reagents Package, DC&trade; Protein Assay Reagents Package, bovine γ-globulin standard; 450 standard assays /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/500-0121_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_view/global/500-0121_view.jpg /webroot/web/images/lsr/products/microplate_systems/sku_feature/global/500-0121_feature.jpg <em>RC DC</em>&trade; Protein Assay Kit I preview RepEx 500-0121 Colorimetric assay kit, includes <em>RC</em> reagents package, <em>DC</em> protein assay reagents package, bovine &#947;-globulin standard; 450 standard assays 500-0121, 5000121, rc, dc, protein, assay, reducing, agent, detergent, compatible, colorimetric, sample, quantitation, concentration 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette N 0 <p>Use the <em>RC DC</em> Protein Assay for samples containing reducing agents and detergents. This assay, based on the <a href="/en-us/product/dc-protein-assay"><em>DC</em> Protein Assay</a>, is a modified form of the Lowry assay that is both reducing agent compatible (<em>RC</em>) and detergent compatible (<em>DC</em>), whereas the <em>DC</em> Assay is only detergent compatible.</p> <ul> <li>Linear over a wide range of protein concentration; standard assay, 0.125&ndash;1.5 mg/ml and low concentration assay, 5&ndash;250 &micro;g/ml</li> <li>Read wavelengths, 650&ndash;750 nm</li> <li>Absorbance values are stable &ge; 1 h</li> <li>Quantitate protein in sample loading buffer prior to gel electrophoresis, either 1-D or 2-D format</li> <li>Determine protein recovery in cell lysates</li> </ul> <p>Sample components compatible with the <em>RC DC</em> Protein Assay include:</p> <table border="0" cellspacing="0" cellpadding="0" width="100%"> <tbody> <tr> <td width="30%" valign="top"> <ul> <li>Ammonium sulfate, 0.5 M</li> <li>&beta;-mercaptoethanol, 10%*</li> <li>Brij 35, 1%</li> <li>C12E8, 0.2%</li> <li>Calcium chloride, 50 mM</li> <li>CHAPS, 2%</li> <li>CHAPSO 1%</li> </ul> </td> <td width="35%" valign="top"> <ul> <li>Dithiothreitol (DTT), 350 mM*</li> <li>EDTA 0.1 M</li> <li>Guanidine hydrochloride, 0.4 M</li> <li>Imidazole, 0.5 M</li> <li>Laemmli buffer (5% &beta;-mercaptoethanol)</li> <li>Nonidet P-40, 2%</li> <li>Octyl-&beta;-glucoside, 1%</li> </ul> </td> <td width="45%" valign="top"> <ul> <li>ReadyPrep&trade; Sequential Extraction Reagents 2 and 3*</li> <li>SDS, 10%</li> <li>Tributylphosphine (TBP), 2 mM</li> <li>Tris, pH 8.4, 0.5 M</li> <li>Triton X-100, 2%</li> <li>Tween 20, 2%</li> <li>Urea, 4 M</li> </ul> </td> </tr> </tbody> </table> <br /> <br /> <p class="caption" style="margin-top:-20px">*Two washes required for highest concentrations</p> <h2 class="descHeader">Product Contents</h2> <ul> <li><em>RC</em> Reagent I, 250 ml</li> <li><em>RC</em> Reagent II, 250 ml</li> <li>Alkaline copper tartrate, 250 ml</li> <li>Dilute Folin reagent, 2 L</li> <li>Surfactant solution, 5 ml</li> <li>Lyophilized bovine &gamma;-globulin, 1 vial</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 450px;" border="0"> <tbody> <tr> <td style="text-align: left;" valign="top"><strong>Kit</strong></td> <td style="text-align: left;" valign="top"><strong>Protein Standard</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><em>RC DC</em> Protein Assay Kit I</td> <td style="text-align: left;" valign="top">Bovine &gamma;-globulin</td> <td style="text-align: center;" valign="top">5000121</td> </tr> <tr> <td style="text-align: left;" valign="top"><em>RC DC</em> Protein Assay Kit II</td> <td style="text-align: left;" valign="top">Bovine serum albumin</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/5000122-rc-dc-protein-assay-kit-ii">5000122 </a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li>Protein Standard I, lyophilized bovine &gamma;-globulin (<a href="/en-us/sku/5000005-protein-standard-i">5000005</a>)</li> <li>Protein Standard II, lyophilized bovine serum albumin (<a href="/en-us/sku/5000007-protein-standard-ii">5000007</a>)</li> <li>Semimicrovolume Disposable Polystyrene Cuvettes (<a href="/en-us/sku/2239955-semimicrovolume-disposable-polystyrene-cuvettes">2239955</a>)</li> <li>Standard Disposable Polystyrene Cuvettes (<a href="/en-us/sku/2239950-standard-disposable-polystyrene-cuvettes">2239950</a>)</li> <li>Protein Assay Reagent A, refill, 250 ml alkaline copper tartrate (<a href="/en-us/sku/5000113-protein-assay-reagent">5000113</a>)</li> <li>Protein Assay Reagent B, refill, 1 L Folin reagent (<a href="/en-us/sku/5000114-protein-assay-reagent-b">5000114</a>)</li> <li>Protein Assay Reagent S, refill, 5 ml surfactant (<a href="/en-us/sku/5000115-protein-assay-reagent-s">5000115</a>)</li> <li>RC Reagent I, refill, 250 ml (<a href="/en-us/sku/5000117-rc-reagent-i">5000117</a>)</li> <li>RC Reagent II, refill, 250 ml (<a href="/en-us/sku/5000118-rc-reagent-ii">5000118</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/product/spectrophotometry/smartspec-plus-spectrophotometer?ID=e12b12ef-e6e2-4ae3-9faa-fd4bfb60ccca">SmartSpec&trade; Plus Spectrophotometer</a></li> <li><a href="/en-us/category/microplate-readers">Microplate Readers</a></li> <li><a href="/en-us/category/general-laboratory-equipment/mixing-devices?ID=MTV0D4C4S">Mixing Devices</a></li> <li><a href="/en-us/product/general-laboratory-equipment/centrifuges">Centrifuges</a></li> <li><a href="/en-us/category/protein-assay-kits-cuvettes">Protein Assay Kits and Cuvettes</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li><em>RC DC</em> Protein Assay <a href="/webroot/web/pdf/lsr/literature/Bulletin_2610D.pdf" target="_blank">Product Information Sheet</a></li> <li><em>RC DC</em> Protein Assay <a href="/webroot/web/pdf/lsr/literature/4110107A.pdf" target="_blank">Instruction Manual</a></li> <li>Colorimetric Protein Assays <a href="/webroot/web/pdf/lsr/literature/Bulletin_1069.pdf" target="_blank">Application Note</a></li> </ul> <h2 class="descHeader">Reference</h2> <p>Lowry OH et al. (1951). Protein Measurement with the Folin Phenol Reagent. J Biol Chem 193, 265-275. PMID: <a href="http://www.ncbi.nlm.nih.gov/pubmed/14907713">14907713</a><br /></p> /webroot/web/html/lsr/products/SKUs/autopopulated 5000121.html /webroot/web/html/lsr/products/SKUs/autopopulated/5000121.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000121&CartQuote=QT&quantity= true true false $314.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000121&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000121&quantity= 500-0121-MSDS DC Universal Protein Assay Kit I Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_5000121.pdf Belgium French /webroot/web/pdf/WWMSDS/LSGC/B/B_FR_5000121.pdf Italy Spanish /webroot/web/pdf/WWMSDS/LSGC/ISL/ISL_ES_5000121.pdf Mexico Spanish /webroot/web/pdf/WWMSDS/LSGC/MEX/MEX_ES_5000121.pdf Russian Federation Russian /webroot/web/pdf/WWMSDS/LSGC/RUS/RUS_RUS_5000121.pdf Hungary English /webroot/web/pdf/WWMSDS/LSGC/HE/HE_ENG_5000121.pdf Hungary Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_5000121.pdf Denmark Danish /webroot/web/pdf/WWMSDS/LSGC/DK/DK_DA_5000121.pdf Germany English /webroot/web/pdf/WWMSDS/LSGC/DE/DE_ENG_5000121.pdf Belgium English /webroot/web/pdf/WWMSDS/LSGC/BE/BE_ENG_5000121.pdf Portugal Portuguese /webroot/web/pdf/WWMSDS/LSGC/P/P_PT_5000121.pdf USA English /webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_5000121.pdf true Sweden Swedish /webroot/web/pdf/WWMSDS/LSGC/S/S_S_5000121.pdf Singapore English /webroot/web/pdf/WWMSDS/LSGC/SGP/SGP_ENG_5000121.pdf United Kingdom English /webroot/web/pdf/WWMSDS/LSGC/GB/GB_ENG_5000121.pdf Austria English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_5000121.pdf France English /webroot/web/pdf/WWMSDS/LSGC/FE/FE_ENG_5000121.pdf New Zealand English /webroot/web/pdf/WWMSDS/LSGC/NZ/NZ_ENG_5000121.pdf Switzerland German /webroot/web/pdf/WWMSDS/LSGC/CH/CH_D_5000121.pdf Italy English /webroot/web/pdf/WWMSDS/LSGC/IE/IE_ENG_5000121.pdf Switzerland English /webroot/web/pdf/WWMSDS/LSGC/CHE/CHE_ENG_5000121.pdf Finland Finnish /webroot/web/pdf/WWMSDS/LSGC/SF/SF_SF_5000121.pdf Israel English /webroot/web/pdf/WWMSDS/LSGC/IL/IL_ENG_5000121.pdf France French /webroot/web/pdf/WWMSDS/LSGC/F/F_FR_5000121.pdf Austria German /webroot/web/pdf/WWMSDS/LSGC/A/A_D_5000121.pdf Switzerland French /webroot/web/pdf/WWMSDS/LSGC/CHF/CHF_FR_5000121.pdf Norway Norwegian /webroot/web/pdf/WWMSDS/LSGC/N/N_N_5000121.pdf Australia English /webroot/web/pdf/WWMSDS/LSGC/AUS/AUS_ENG_5000121.pdf Canada French /webroot/web/pdf/WWMSDS/LSGC/FCN/FCN_FR_5000121.pdf The Netherlands Dutch /webroot/web/pdf/WWMSDS/LSGC/NL/NL_NL_5000121.pdf Germany German /webroot/web/pdf/WWMSDS/LSGC/D/D_D_5000121.pdf Czech Republic Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_5000121.pdf United Arab Emirates English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_5000121.pdf Denmark English /webroot/web/pdf/WWMSDS/LSGC/DKE/DKE_ENG_5000121.pdf Poland Polish /webroot/web/pdf/WWMSDS/LSGC/PL/PL_POL_5000121.pdf Hong Kong English /webroot/web/pdf/WWMSDS/LSGC/HK/HK_ENG_5000121.pdf Italy Italian /webroot/web/pdf/WWMSDS/LSGC/I/I_IT_5000121.pdf Canada English /webroot/web/pdf/WWMSDS/LSGC/CDN/CDN_USA_5000121.pdf China Chinese /webroot/web/pdf/WWMSDS/LSGC/RC/RC_ZH_5000121.pdf Spain Spanish /webroot/web/pdf/WWMSDS/LSGC/E/E_ES_5000121.pdf Japan Japanese /webroot/web/pdf/WWMSDS/LSGC/J/J_J_5000121.pdf Czech Republic English /webroot/web/pdf/WWMSDS/LSGC/CZE/CZE_ENG_5000121.pdf Sweden English /webroot/web/pdf/WWMSDS/LSGC/SE/SE_ENG_5000121.pdf France Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_5000121.pdf France Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_5000121.pdf China English /webroot/web/pdf/WWMSDS/LSGC/PRC/PRC_ENG_5000121.pdf India English /webroot/web/pdf/WWMSDS/LSGC/IN/IN_ENG_5000121.pdf Korea English /webroot/web/pdf/WWMSDS/LSGC/KOR/KOR_ENG_5000121.pdf Japan English /webroot/web/pdf/WWMSDS/LSGC/NIP/NIP_USA_5000121.pdf Poland English /webroot/web/pdf/WWMSDS/LSGC/POL/POL_ENG_5000121.pdf The Netherlands English /webroot/web/pdf/WWMSDS/LSGC/NLE/NLE_ENG_5000121.pdf
List Price: 
 
$314.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
Hazardous  

Use the RC DC Protein Assay for samples containing reducing agents and detergents. This assay, based on the DC Protein Assay, is a modified form of the Lowry assay that is both reducing agent compatible (RC) and detergent compatible (DC), whereas the DC Assay is only detergent compatible.

  • Linear over a wide range of protein concentration; standard assay, 0.125–1.5 mg/ml and low concentration assay, 5–250 µg/ml
  • Read wavelengths, 650–750 nm
  • Absorbance values are stable ≥ 1 h
  • Quantitate protein in sample loading buffer prior to gel electrophoresis, either 1-D or 2-D format
  • Determine protein recovery in cell lysates

Sample components compatible with the RC DC Protein Assay include:

  • Ammonium sulfate, 0.5 M
  • β-mercaptoethanol, 10%*
  • Brij 35, 1%
  • C12E8, 0.2%
  • Calcium chloride, 50 mM
  • CHAPS, 2%
  • CHAPSO 1%
  • Dithiothreitol (DTT), 350 mM*
  • EDTA 0.1 M
  • Guanidine hydrochloride, 0.4 M
  • Imidazole, 0.5 M
  • Laemmli buffer (5% β-mercaptoethanol)
  • Nonidet P-40, 2%
  • Octyl-β-glucoside, 1%
  • ReadyPrep™ Sequential Extraction Reagents 2 and 3*
  • SDS, 10%
  • Tributylphosphine (TBP), 2 mM
  • Tris, pH 8.4, 0.5 M
  • Triton X-100, 2%
  • Tween 20, 2%
  • Urea, 4 M


*Two washes required for highest concentrations

Product Contents

  • RC Reagent I, 250 ml
  • RC Reagent II, 250 ml
  • Alkaline copper tartrate, 250 ml
  • Dilute Folin reagent, 2 L
  • Surfactant solution, 5 ml
  • Lyophilized bovine γ-globulin, 1 vial

Packaging Options

Kit Protein Standard Catalog Number
RC DC Protein Assay Kit I Bovine γ-globulin 5000121
RC DC Protein Assay Kit II Bovine serum albumin 5000122

Related Products

  • Protein Standard I, lyophilized bovine γ-globulin (5000005)
  • Protein Standard II, lyophilized bovine serum albumin (5000007)
  • Semimicrovolume Disposable Polystyrene Cuvettes (2239955)
  • Standard Disposable Polystyrene Cuvettes (2239950)
  • Protein Assay Reagent A, refill, 250 ml alkaline copper tartrate (5000113)
  • Protein Assay Reagent B, refill, 1 L Folin reagent (5000114)
  • Protein Assay Reagent S, refill, 5 ml surfactant (5000115)
  • RC Reagent I, refill, 250 ml (5000117)
  • RC Reagent II, refill, 250 ml (5000118)

Related Categories

Supporting Documentation

Reference

Lowry OH et al. (1951). Protein Measurement with the Folin Phenol Reagent. J Biol Chem 193, 265-275. PMID: 14907713

Available Translation
DC Universal Protein Assay Kit I  (SDS)gene

Bulletin# 500-0121-MSDS

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Get Help
Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.