The iQ-Check E. coli O157:H7 Kit is a rapid method consisting of ready-to-use reagents for real-time PCR amplification and detection of E. coli O157:H7 in food samples. Each kit has enough reagents to process up to 96 samples. The kit contains grinding beads, lysis reagent, PCR mix, a positive control, and a negative control. Each reaction has an internal amplification control to validate a negative result. After a single primary 8–24 hr enrichment, samples are ready for DNA extraction and real-time PCR analysis.