The iQ-Check Salmonella II Kit is a rapid method consisting of ready-to-use reagents for real-time PCR amplification and detection of Salmonella spp. in food and environmental samples. Each kit has enough reagents to process up to 96 samples. The kit contains lysis reagent, PCR mix, a positive control, and a negative control. Each reaction has an internal amplification control to validate a negative result. After a single primary 20 hr enrichment in non-selective buffered peptone water, samples are ready for DNA extraction and real-time PCR analysis.