RAPID'L.mono™ medium is a chromogenic medium for detection, enumeration, and differentiation of Listeria spp., specifically Listeria monocytogenes from food and environmental samples. This kit contains one 190 ml bottle of agar and two supplements. Supplement 1 contains 6 ml of liquid, while Supplement 2 is lyophilized and has to be rehydrated with 14 ml of sterile distilled water. Agar is melted and tempered, then supplements are added. Complete agar is poured into petri dishes. One kit generates approximately 13 plates, assuming each plate is poured with 15 ml of agar.