3–8% Criterion™ XT Tris-Acetate Protein Gel, 11 cm IPG/prep+1 well, 550 µl #3450133

3–8% Criterion™ XT Tris-Acetate Protein Gel, 11 cm IPG/prep+1 well, 550 µl

Pkg of 1, 3-8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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Gel percentage 3–8
Comb 11 cm IPG+1-well
Shelf life, months* 8
Gel storage conditions Store flat at 2–8°C; do not freeze
Gel dimensions (W x L x thickness) (cm) 13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness) (cm) 15.0 x 10.6 x 0.53
Cassette material Styrene copolymer
Comb material Polycarbonate
Storage tray material PET
* From date of manufacture.  
Number Description Options
345-0129-MSDS Criterion™ XT Tris-Acetate Gel English

Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without SDS, which allow the sample buffer and running buffer to determine the separation mechanism.

  • For native PAGE, use Native Sample and Tris/Glycine Native Running Buffers
  • For separation by molecular weight, use XT Sample and XT Tricine Running Buffers
  • Neutral pH (pH 7.0) delays acrylamide hydrolysis as compared to traditional Laemmli systems
  • 8-month shelf life
  • Run 2-D gels in less than a day
  • Separation range is ~40–400 kD under denaturing conditions
  • One well available for a marker or other reference

Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate proteins by both size and charge.

Packaging Options

Criterion XT Tris-Acetate Midi Protein Gels are available as 3–8% (separation range ~40–400 kD) or 7% (separation range ~35–250 kD) gels, with a selection of different well configurations.

Well Configuration, Volume Catalog Number
3–8% Midi
12+2 well, 45 µl 3450129 3450135
18 well, 30 µl 3450130 3450136
26 well, 15 µl 3450131 3450137
11 cm IPG/prep+1 well, 550 µl 3450133

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