12.5% Criterion™ Zymogram Gel with Casein, 18 well, 30 µl #3450083


Number Description Options
345-0084-MSDS Criterion™ Zymogram Ready Gel® English

Use midi 12.5% Criterion Zymogram Gels with Casein to test for proteolytic activity. After electrophoresis under nonreducing conditions, enzymes in the sample are renatured, and the casein within the gel is proteolyzed. After digestion, the gels are most frequently stained with Coomassie Brilliant Blue R-250 stain, which stains the substrate. Active proteases will have a clear area around them where proteolysis has occurred.

  • For analysis of  proteases including matrix metalloproteinases (MMPs)
  • Samples are denatured in SDS, but not reduced nor heated
  • Available with casein or gelatin
  • Provided in single-use cassettes

Packaging Options

Gel Percentage Substrate Optimum Separation Range Well Number and Configuration, Volume
Catalog Number
12+2 well, 45 µl 18 well, 30 µl 26 well, 15 µl
10% Gelatin 30–150 kD 3450079 3450080 3450081
12.5% Casein 20–120 kD 3450082 3450083 3450084


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  • 10x Tris/Glycine/SDS (1610772)
  • Coomassie Brilliant Blue R-250 Staining Solution (1610436)
  • Coomassie Brilliant Blue R-250 Destaining Solution (1610438)
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