10% Criterion™ Zymogram Gel with Gelatin, 18 well, 30 µl #3450080


Number Description Options
345-0081-MSDS 10% Criterion™ Zymogram Ready Gel® English
345-0084-MSDS Criterion™ Zymogram Ready Gel® English

Use midi 10% Criterion Zymogram Gels with Gelatin to test for proteolytic activity. After electrophoresis under nonreducing conditions, enzymes in the sample are renatured, and the gelatin within the gel is proteolyzed. After digestion, the gels are most frequently stained with Coomassie Brilliant Blue R-250 stain, which stains the substrate. Active proteases will have a clear area around them where proteolysis has occurred.

  • For analysis of proteases including matrix metalloproteinases (MMPs)
  • Samples are denatured in SDS, but not reduced nor heated
  • Available with casein or gelatin
  • Provided in single-use cassettes

Packaging Options

Gel Percentage Substrate Optimum Separation Range Well Number and Configuration, Volume
Catalog Number
12+2 well, 45 µl 18 well, 30 µl 26 well, 15 µl
10% Gelatin 30–150 kD 3450079 3450080 3450081
12.5% Casein 20–120 kD 3450082 3450083 3450084

Related Products

  • Zymogram Sample Buffer (1610764)
  • 10x Zymogram Renaturation Buffer (1610765)
  • 10x Zymogram Development Buffer (1610766)
  • 10x Tris/Glycine/SDS (1610772)
  • Coomassie Brilliant Blue R-250 Staining Solution (1610436)
  • Coomassie Brilliant Blue R-250 Destaining Solution (1610438)
  • Criterion Cell — for one or two vertical, midi-format gels (1656001)
  • Criterion Dodeca Cell — for up to twelve vertical, midi-format gels (1654130)
  • Criterion Staining/Blotting Trays (3459921)