10% Criterion™ Zymogram Gel with Gelatin, 12+2 well, 45 µl #3450079

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Number Description Options
345-0081-MSDS 10% Criterion™ Zymogram Ready Gel® English
345-0084-MSDS Criterion™ Zymogram Ready Gel® English

Use midi 10% Criterion Zymogram Gels with Gelatin to test for proteolytic activity. After electrophoresis under nonreducing conditions, enzymes in the sample are renatured and then the gelatin within the gel is proteolyzed. After digestion, the gels are most frequently stained with Coomassie Brilliant Blue R-250 stain, which stains the substrate. Active proteases will have a clear area around them where proteolysis has occurred.

  • For analysis of  proteases including matrix metalloproteinases (MMPs)
  • Two wells for markers or other references
  • Samples are denatured in SDS, but generally not reduced or heated
  • Gels available with either casein or gelatin
  • Precast Criterion TBE Gels are provided in a single-use cassettes

Packaging Options

Gel Percentage Substrate Optimum Separation Range Well Number and Configuration, Volume
Catalog Number
12+2 well, 45 µl 18 well, 30 µl 26 well, 15 µl
10% Gelatin 30–150 kD 3450079 3450080 3450081
12.5% Casein 20–120 kD 3450082 3450083 3450084

 

Related Products

  • Zymogram Sample Buffer (1610764)
  • 10x Zymogram Renaturation Buffer (1610765)
  • 10x Zymogram Development Buffer (1610766)
  • 10x Tris/Glycine/SDS (1610772)
  • Coomassie Brilliant Blue R-250 Staining Solution (1610436)
  • Coomassie Brilliant Blue R-250 Destaining Solution (1610438)
  • Criterion Cell — for one or two vertical, midi-format gels (1656001)
  • Criterion Dodeca Cell — for up to twelve vertical, midi-format gels (1654130)
  • Criterion™ Staining/Blotting Trays (3459921)