QX200 AutoDG Droplet Digital PCR System #1864100

The QX200 AutoDG Droplet Digital PCR System simplifies the Droplet Digital PCR (ddPCR) workflow, reducing hands-on time and eliminating user-to-user variability. The AutoDG Instrument brings scalability and flexibility to Bio-Rad’s ddPCR technology, the most precise and sensitive digital PCR solution for the absolute quantification of target DNA molecules.

• Eliminates user-to-user variability — creates more consistent droplet counts across users and plates, enabling you to fully exploit the precision of your ddPCR System
• Minimizes hands-on time — takes less than 5 minutes to load the instrument, and droplet generation requires no supervision

Features of the QX200 AutoDG Droplet Digital PCR system

AutoDG Instrument:

• Guides setup and loading with large, color touch-screen interface and LED lighting
• Compatible with both the QX100 and QX200 Droplet Digital PCR Systems
• Generates droplets for 96 ddPCR reactions in under 45 minutes
• One AutoDG Instrument can supply 4–5 droplet readers continuously
• HEPA-filtered enclosure reduces contamination and allows the system to be placed in any lab environment
• User-definable plate layout with increments of 8 wells provides run-to-run flexibility

Droplet Digital PCR Technology:

• Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
• Droplet partitioning by the QX200 Droplet Digital PCR system reduces bias from amplification efficiency and PCR inhibitors
• Convenient assay design — standard curves are not required

Learn more about the applications of the QX200™ AutoDG™ Droplet Digital™ PCR System.

Required Instruments

The Automated Droplet Generator requires the use of the following Bio-Rad instruments:

• C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module
• PX1 PCR Plate Sealer

Required Consumables

• DG32 Automated Droplet Generator Cartridges for the AutoDG System
• Automated Droplet Generation Oil for Probes
• Automated Droplet Generation Oil for EvaGreen
• Pipet Tips for the AutoDG System
• Pipet Tip Waste Bin for the AutoDG System
• Droplet Reader Oil or ddPCR Droplet Reader Oil EcoTank for Probes

ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications

• PrimePCR ddPCR Assays
• ddPCR Supermixes for Probes (no dUTP)
• QX200 ddPCR EvaGreen Supermix
• ddPCR Supermix for Probes
• One-Step RT-ddPCR Kit for Probes

Supporting Documents and Resources

QX200 AutoDG Droplet Digital PCR System Instruction Manual

Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including: Copy number variation analysis Rare mutation and sequence detection Gene expression Next-generation sequencing library analysis