One-Step RT-ddPCR Kit for Probes #186-3022

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5 ml (5 x 1 ml), 500 x 20 µl reactions, 2x RT-ddPCR Mix, includes 2 manganese acetate tubes, for use with QX200™/QX100™ Droplet Digital™ PCR Systems

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Storage at –20°C Up to 12 months (refer to expiration date)
Storage at 4°C (after thawing) Up to 2 weeks
Template compatibility RNA, synthetic RNA

Use this 2x One-Step RT-ddPCR Supermix Kit to perform RNA reverse transcription and subsequent amplification in the same reaction.

Key Benefits

  • Contains all components, except primers, probes, and template, required for target-specific RT-ddPCR
  • Enhances the specificity and efficiency of primer-mediated cDNA conversion by performing the reverse transcription reaction at 55–60°C
  • Contains RNase inhibitor that protects the RNA throughout the workflow
  • Suitable for UNG decontamination protocols

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 186-3021
500 5 x 1 186-3022


Additional Reagents

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

This digital PCR supermix requires the use of the following instruments and accessories:

Related Products

Bio-Rad offers additional digital PCR supermixes including:

More Information

This One-Step ddPCR Supermix for Probes is optimized for use with Droplet Generation Oil for Probes on the QX200™ Droplet Digital™ PCR System.