CFX384™ Optical Reaction Module for Real-Time PCR Systems #1845385

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384-well optical module for real-time PCR, includes cables, for use with C1000 Touch™ Thermal Cycler Chassis

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The 384-well optical module includes an optical system for collection of fluorescent data and a thermal cycler block.</p> <p>The CFX384 Optical Reaction Module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Chassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.</p> <h2 class="descHeader">Features and Benefits</h2> <p>When the CFX384 Optical Reaction Module is combined with the thermal cycler chassis, the system offers:</p> <ul> <li>Detection of up to 4 targets per well, plus a channel dedicated to singleplex FRET</li> <li>Protocol autowriter that generates an optimal protocol for your reaction components</li> <li>Thermal gradient feature that identifies optimal annealing temperature in a single run</li> </ul> <p>The CFX384 Touch&trade; Real-Time PCR Detection System has six independently controlled thermal electric units, providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity).</p> <p>The <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optical_cfx_touch.html&quot;);">optical system</a>, with five filtered LEDs and five filtered photodiodes, collects data from all wells, detecting up to four targets per well. For single-color FAM and SYBR<sup>&reg;</sup> Green I the fast scan option reads single-channel fluorescence in all 384 wells in just 8 seconds. A channel with an LED filter&ndash;photodiode combination is dedicated to FRET singleplex experiments.</p> <p>The large touch-screen interface has intuitive graphical programming and displays data in real time. The programmable thermal gradient feature can test eight temperatures at once to quickly determine the optimal annealing temperature, and the protocol autowriter quickly optimizes reactions, generating an optimal protocol based on your polymerase, primers, and product length.</p> <h2 class="descHeader">Product Components</h2> <ul> <li>CFX384 Optical Reaction Module </li> <li>USB cable</li> <li><a href="/en-us/product/cfx-manager-software"></a>Instruction manual</li> <li>SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix (<a href="/en-us/sku/1725270-ssoadvanced-universal-sybr-green-supermix">1725270</a>)</li> <li>Hard-Shell<sup>&reg;</sup> Thin-Wall 384-Well Skirted PCR Plates (<a href="/en-us/sku/hsp3805-hard-shell-thin-wall-384-well-skirted-pcr-plates">HSP3805</a>)</li> <li>Microseal<sup>&reg;</sup> 'B' Adhesive Seals (<a href="/en-us/sku/msb1001-microseal-b-adhesive-seals">MSB1001</a>)</li> </ul> <h2 class="descHeader">Configuration Options</h2> <p>The following modules can be used with the C1000 Touch Thermal Cycler Chassis:<br /></p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="319" valign="middle"><strong>Reaction Modules</strong></td> <td style="text-align: center;" colspan="2" valign="center"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">&nbsp;</td> <td style="text-align: center;" width="319" valign="top"><strong>Module</strong></td> <td style="text-align: center;" width="319" valign="top"><strong>Module with Starter Package*</strong></td> </tr> <tr> <td valign="top"><strong>For PCR</strong></td> <td style="text-align: center;" valign="top"><br /></td> <td style="text-align: center;" valign="top">&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td valign="top">Dual 48/48 Fast Reaction Module</td> <td style="text-align: center;" valign="middle"><a href="/en-us/sku/1840148-dual-48-48-fast-reaction-module">1840148</a></td> <td style="text-align: center;" valign="middle">&mdash;</td> </tr> <tr> <td valign="top">96-Well Fast Reaction Module</td> <td style="text-align: center;" valign="middle"><a href="/en-us/sku/1840196-96-well-fast-reaction-module">1840196</a></td> <td style="text-align: center;" valign="middle">&mdash;</td> </tr> <tr class="pd_colorbackground"> <td valign="top">96&ndash;Deep Well Reaction Module</td> <td style="text-align: center;" valign="middle"><a href="/en-us/sku/1840197-96-ndash-deep-well-reaction-module">1840197</a></td> <td style="text-align: center;" valign="middle">&mdash;</td> </tr> <tr> <td valign="top">384-Well Reaction Module</td> <td style="text-align: center;" valign="middle"><a href="/en-us/sku/1840138-384-well-reaction-module">1840138</a></td> <td style="text-align: center;" valign="middle">&mdash;</td> </tr> <tr class="pd_colorbackground"> <td valign="top"><strong>For Real-Time PCR</strong></td> <td style="text-align: center;" valign="middle">&mdash;</td> <td style="text-align: center;" valign="middle">&mdash;</td> </tr> <tr> <td valign="top">CFX96&trade; Optcal Reacton Module<br /></td> <td style="text-align: center;" valign="middle"><a href="/en-us/sku/1845097-cfx96-optical-reaction-module">1845097</a></td> <td style="text-align: center;" valign="middle"><a href="/en-us/sku/1845096-cfx96-optical-reaction-module">1845096</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">CFX96 Deep Well Optical Reaction Module<br /></td> <td style="text-align: center;" valign="middle"><a href="/en-us/sku/1844095-cfx96-deep-well-optical-reaction-module">1844095</a></td> <td style="text-align: center;" valign="middle"><a href="/en-us/sku/1844096-cfx96-deep-well-optical-reaction-module">1844096</a></td> </tr> <tr> <td valign="top">CFX384&trade; Optical Reacton Module</td> <td style="text-align: center;" valign="middle">1845385</td> <td style="text-align: center;" valign="middle"><a href="/en-us/sku/1845384-cfx384-optical-reaction-module">1845384</a></td> </tr> </tbody> </table> <br /><br /> <p class="caption" style="margin-top:-20px">*Starter Package contains SsoAdvanced Universal SYBR Green Supermix (<a href="/en-us/sku/1725270-ssoadvanced-universal-sybr-green-supermix">1725270</a>), Microseal 'B' Adhesive Seals (<a href="/en-us/sku/msb1001-microseal-b-adhesive-seals">MSB1001</a>), and Hard-Shell Low-Profile Thin-Wall 96-Well Skirted PCR Plates (<a href="/en-us/sku/hsp9601-hard-shell-low-profile-thin-wall-96-well-skirted-pcr-plates">HSP9601</a>) or Hard-Shell Thin-Wall 384-Well Skirted PCR Plates (<a href="/en-us/sku/hsp3805-hard-shell-thin-wall-384-well-skirted-pcr-plates">HSP3805</a>).</p> <p class="caption" style="margin-top:-20px">&nbsp;</p> <p>The CFX384 Touch Real-Time PCR Detection System can be configured with other PCR instruments, allowing stand-alone or software-controlled systems. <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/deepwell_flex.html&quot;);">Instrument permutations</a> allow up to four different CFX Connect&trade;, CFX96, or CFX384 Systems to be controlled by one PC.</p> <p>Combine any two CFX Real-Time PCR Detection Systems with the <a href="/en-us/product/cfx-automation-system-ii">CFX Automation System II</a>, our robotic plate handler, to create a high-throughput, automated real-time PCR system with a compact footprint.</p> <div id="cfxPrimePCR"> <script type="text/javascript"><!-- $('#cfxPrimePCR').load('/webroot/web/html/lsr/products/amplification_pcr/cfx-primepcr.html'); // --></script> </div> <h2 class="descHeader">Service Plans and Validation Tools for CFX Systems</h2> <ul> <li>Ensure that your real-time PCR systems are functioning optimally with <a href="/en-us/life-science-research/purchase-service-programs/expert-care-service">Bio-Rad Expert Care Service</a>; choose from several service plans to keep your CFX Automation System II running its best</li> <li>Certify system installation and validate system performance with <a href="/en-us/product/iq-oq-services">CFX System IQ/OQ Services</a> and <a href="/en-us/sku/1845082-iq-oq-kit-service-for-cfx-automation-system-ii">CFX Automation System II IQ/OQ Services </a></li> <li>Verify the performance of your CFX Systems with <a href="/en-us/product/thermal-validation-services">Thermal Validation Services</a> and the <a href="/en-us/product/cfx-qualification-plate">CFX Qualification Plates </a></li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li>C1000 Touch Thermal Cycler Chassis (<a href="/en-us/sku/1841100-c1000-touch-thermal-cycler-chassis">1841100</a>)</li> <li><a href="/en-us/product/px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer</a> </li> <li>PCR plastics optimized for CFX Real-Time PCR Detection Systems: <ul> <li><a href="/en-us/product/384-well-plates-for-pcr/hard-shell-384-well-skirted-pcr-plates">Hard-Shell 384-Well Skirted PCR Plates </a></li> <li>Microseal 'B' Adhesive Seals (<a href="/en-us/sku/msb1001-microseal-b-adhesive-seals">MSB1001</a>)</li> <li>Optically Clear Heat Seals (<a href="/en-us/sku/1814030-optically-clear-heat-seal">1814030</a>)</li> </ul> </li> <li style="margin-top:-9px"> <a href="/en-us/sku/12005258-cfx-maestro-software-security-edition-1-license">CFX Maestro Software, Security Edition</a>, for compliance with U.S. FDA 21 CFR Part 11 regulations </li> <li>CFX Qualification Plate for performance verification, 384-well&nbsp; (<a href="/en-us/sku/1845099-cfx-qualification-plate">1845099</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a> </li> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR Reagents and qPCR Reagents </a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables </a></li> <li><a href="/en-us/category/pcr-seals">PCR Seals </a></li> <li><a href="/en-us/category/pcr-analysis-software">PCR Analysis Software</a> </li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>CFX384 Touch Real-Time PCR Detection System <a href="/webroot/web/pdf/lsr/literature/Bulletin_6072.pdf" target="_blank">Specifications Sheet </a></li> <li>CFX384 Touch Real-Time PCR System Installation <a href="/webroot/web/pdf/lsr/literature/10021419.pdf" target="_blank">Quick Guide</a> </li> <li>CFX96 Touch, CFX96 Touch Deep Well, and CFX384 Touch Real-Time PCR Detection Systems <a href="/webroot/web/pdf/lsr/literature/10000068706.pdf" target="_blank">Instruction Manual </a></li> <li>CFX Qualification Plate &mdash; 384-Well Plate Format <a href="/webroot/web/pdf/lsr/literature/10027342.pdf" target="_blank">Product Insert </a></li> <li>Real-Time PCR <a href="/webroot/web/pdf/lsr/literature/Bulletin_5279.pdf" target="_blank">Applications Guide</a> </li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li>Real-Time PCR Detection Systems <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/amp_realtime_pcr_systms.html&quot;);">Selection Chart</a></li> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a> </li> <li><a href="/en-us/applications-technologies/qpcr-instrumentation">qPCR Instrumentation</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1845385.html /webroot/web/html/lsr/products/SKUs/autopopulated/1845385.html &nbsp; 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1845385&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1845385&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1845385&quantity=
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Use the CFX384 Optical Reaction Module for precise, reproducible real-time PCR. The 384-well optical module includes an optical system for collection of fluorescent data and a thermal cycler block.

The CFX384 Optical Reaction Module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Chassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.

Features and Benefits

When the CFX384 Optical Reaction Module is combined with the thermal cycler chassis, the system offers:

  • Detection of up to 4 targets per well, plus a channel dedicated to singleplex FRET
  • Protocol autowriter that generates an optimal protocol for your reaction components
  • Thermal gradient feature that identifies optimal annealing temperature in a single run

The CFX384 Touch™ Real-Time PCR Detection System has six independently controlled thermal electric units, providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity).

The optical system, with five filtered LEDs and five filtered photodiodes, collects data from all wells, detecting up to four targets per well. For single-color FAM and SYBR® Green I the fast scan option reads single-channel fluorescence in all 384 wells in just 8 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.

The large touch-screen interface has intuitive graphical programming and displays data in real time. The programmable thermal gradient feature can test eight temperatures at once to quickly determine the optimal annealing temperature, and the protocol autowriter quickly optimizes reactions, generating an optimal protocol based on your polymerase, primers, and product length.

Product Components

  • CFX384 Optical Reaction Module
  • USB cable
  • Instruction manual
  • SsoAdvanced™ Universal SYBR® Green Supermix (1725270)
  • Hard-Shell® Thin-Wall 384-Well Skirted PCR Plates (HSP3805)
  • Microseal® 'B' Adhesive Seals (MSB1001)

Configuration Options

The following modules can be used with the C1000 Touch Thermal Cycler Chassis:

Reaction Modules Catalog Number
  Module Module with Starter Package*
For PCR
 
Dual 48/48 Fast Reaction Module 1840148
96-Well Fast Reaction Module 1840196
96–Deep Well Reaction Module 1840197
384-Well Reaction Module 1840138
For Real-Time PCR
CFX96™ Optcal Reacton Module
1845097 1845096
CFX96 Deep Well Optical Reaction Module
1844095 1844096
CFX384™ Optical Reacton Module 1845385 1845384


*Starter Package contains SsoAdvanced Universal SYBR Green Supermix (1725270), Microseal 'B' Adhesive Seals (MSB1001), and Hard-Shell Low-Profile Thin-Wall 96-Well Skirted PCR Plates (HSP9601) or Hard-Shell Thin-Wall 384-Well Skirted PCR Plates (HSP3805).

 

The CFX384 Touch Real-Time PCR Detection System can be configured with other PCR instruments, allowing stand-alone or software-controlled systems. Instrument permutations allow up to four different CFX Connect™, CFX96, or CFX384 Systems to be controlled by one PC.

Combine any two CFX Real-Time PCR Detection Systems with the CFX Automation System II, our robotic plate handler, to create a high-throughput, automated real-time PCR system with a compact footprint.

Service Plans and Validation Tools for CFX Systems

Related Products

Related Categories

Supporting Documents

Online Resources

 
Number Description Options

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