Use ROX Passive Reference Dye on real-time thermal cyclers that require an internal reference dye for fluorescent signal normalization and correction of well-to-well optical variations. ROX passive reference dye allows seamless integration of non-ROX-containing PCR reagents on all ROX-dependent real-time instrument systems.
Since the excitation optics of different instrument platforms vary, the optimal reference dye concentration must be matched to the given real-time cycler.
Use ROX Passive Reference Dye as a 50x concentrated stock solution on the ABI PRISM 7000, 7300, 7700, or 7900 systems.
For instruments that use 580–585 nm excitation for passive reference, such as the Stratagene Mx4000, Mx3000P, or Mx3005P, or the ABI PRISM 7500 sequence detection system, the ROX passive reference dye should be treated as a 750x concentrated solution.
An internal reference dye is not required for use with the iCycler iQ®, MyiQ™, DNA Engine® Opticon 2, or Chromo4™ real-time detection systems.