SsoAdvanced™ Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml) #1725275


50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 5,000 x 20 µl reactions

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Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 3 months
Customer guarantee (minimum usage time) at –20°C 12 months
Number Description Options
172-5270-MSDS SsoAdvanced™ Universal SYBR® Green Supermix English

SsoAdvanced™ universal SYBR® Green supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.

SsoAdvanced™ universal SYBR® Green supermix lets you:

  • Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
  • Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
  • Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
  • Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
  • Obtain better results with Bio-Rad's PrimePCR™ assays — real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance

Applications and Uses of SsoAdvanced™ Universal SYBR® Green Supermix

Packaging Options

Number of 20 μl Reactions Total Volume Number of Vials Catalog #
   200   2 ml   2 x 1 ml #172-5270
   500   5 ml   5 x 1 ml #172-5271
1,000 10 ml 10 x 1 ml #172-5272
2,500 25 ml   5 x 5 ml #172-5274
5,000 50 ml 10 x 5 ml #172-5275


Instrument Compatibility

The SsoAdvanced™ universal SYBR® Green supermix is compatible with all commercially available and all Bio-Rad real-time qPCR systems.

Related Products

We have developed SsoAdvanced universal probes supermix, which is available in several formats (200–5,000 reactions).

Bio-Rad offers a complete workflow solution for real-time PCR, including:

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.