iTaq™ Universal Probes One-Step Kit, 500 x 20 µl reactions #172-5141

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500 x 20 µl reactions, 5 ml (5 x 1 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

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Product expiration (after shipping date) 12 months
Reaction mix storage at 4°C (after thawing) Up to 3 months
RT storage at –20°C (after shipping date) 12 months
Customer guarantee (minimum usage time) at –20°C 12 months
Number Description Options
172-5140-MSDS iTaq™ Universal Probes One-Step Kit English

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Use our combined iScript RNase H+ reverse transcriptase and antibody hot-start iTaq DNA polymerase probe kit formulation to complete your real-time reactions in one step — PCR efficiency is improved over a wide dynamic range and under various conditions.

  • Advanced formulation for amplifying difficult target sequences and challenging samples
  • Perform singleplex or multiplex reactions simultaneously
  • Normalize qPCR data with ROX normalization dyes

Kit Components

  • Reverse transcriptase — 250 µl
  • Reaction mix — 5 ml (5 x 1 ml)

Applications and Uses of the iTaq Universal Probes One-Step Kit

  • qPCR/real-time PCR
  • Gene expression analysis
  • Multiplexing
  • Absolute quantification
  • Mutation detection
  • Pathogen detection
  • Viral detection (load)
  • Genetically modified organisms (GMO) characterization
  • Genetic profiling

Packaging Options

The iTaq universal probes one-step kit is also available in a format of 100 reactions (172-5140).

Instrument Compatibility

The iTaq universal probes one-step kit is compatible with all real-time qPCR systems.

Related Products

Bio-Rad has also developed one-step kits for SYBR® Green–specific applications.