(Discontinued) DCode System for DGGE #1709083


120 V, universal mutation detection system, for 10 cm gels with two 1 mm prep wells, includes comb gasket, 2 sets of clamps, Model 475 gradient former with all parts required to cast gradient gels

This product is no longer available. Please contact technical support for more information on replacement product.

The DCode universal mutation detection system has a vertical electrophoresis cell with adaptors for different techniques for identification of single-base changes, providing a simple, efficient, and flexible system for screening and identifying polymorphisms. Use the same system for high-throughput screening for a mutation or hunting for unknowns.

The DCode system can identify single-base changes using any combination of the following electrophoretic techniques:

  • Single-stranded conformation polymorphism (SSCP)
  • Denaturing gradient gel electrophoresis (DGGE)
  • Constant denaturing gel electrophoresis (CDGE)
  • Temporal temperature gradient gel electrophoresis (TTGE)

A unique feature of the DCode system is the ability to perform TTGE. This type of analysis, codeveloped by Bio-Rad, has the advantage of not using a denaturing chemical gradient gel, as is required for DGGE and to optimize CDGE. The denaturing chemical gradient is replaced with a linear temperature gradient, eliminating the need for casting gradient gels. TTGE provides faster, simpler and increased reproducibility of detection of single-base changes.

Features of the DCode universal mutation detection system:

  • Run up to 64 samples in as little as 2 hours
  • Accurate temperature control options for electrophoresis runs between 5° and 70°
  • Temperature ramp as low as 0.1°C/hr
  • MacMelt and WinMelt software help determine optimal temperature ranges, as well as GC clamp and primer placement
  • Gradients may be formed parallel or perpendicular to the direction of electrophoresis
  • Modular design for customizing current and future laboratory demands
  • Different plate dimensions available, including 20 x 16, 16 x 16, 10 x 16 and 7.5 x 16 cm
  • Depending on plate dimensions, run up to four gels simultaneously
  • Specialized accessory kits that simplify startup
  • Technique-specific reagents and controls that are optimized for DGGE, CDGE, SSCP, and TTGE

Training Guide

A self-training guide, an interactive CD-ROM, is available. The guide has videos on setting up and using the DCode system, the principles of SSCP, DGGE, CDGE and TTGE, software tutorials , instruction manual, plus application notes and other reference materials. Request catalog #170-9241. The CD-ROM is PC-compatible only.

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