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Overview
5 agarose blocks, PFGE standard, lambda (λ) ladder, 48.5-1000 kb in 48.5 kb increments, concatemers of λcl857Sam7 lambda DNA per block, for 25–40 plugs
Safety Data Sheet
Description
Description
Use CHEF* DNA size standards for size estimation of larger DNA fragments separated by pulsed field electrophoresis.
- Size range: 48.5–1,000 kb in 48.5 kb increments (λcl857Sam7 concatemers)
- Recommended gels: 0.8–1.0% Pulsed Field Certified Agarose or molecular biology grade agarose
Note: Cut a plug from a block into a size that will fit a well of your gel (5–8 plugs per block). The height of the plug should not extend above the well. After placing the plug into a well, seal the well with melted 1.0% low-melt agarose, and allow it to solidify. Store blocks at 4°C.
| Pulsed Field Size Standards | Size Range |
| CHEF DNA Size Marker (1703605) | 0.2–2.2 Mb |
| CHEF DNA Size Standard (1703624) | 4.9–98 kb |
| CHEF DNA Size Standard (1703635) | 48.5–1,000 kb |
| CHEF DNA Size Marker (1703667) | 1–3.1 Mb |
| CHEF DNA Size Standard (1703707) | 8.3–48.5 kb |
Related Products
- Buffers and Reagents for Nucleic Acid Electrophoresis
- Nucleic Acid Stains and Tracking Dyes
- All Nucleic Acid Standards
More Information
Download the Little Book of Standards for complete product information on all Bio-Rad nucleic acid and protein standards.
*Clamped homogenous electrical field