TransFectin™ Lipid Reagent, 5 ml #1703352

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5 x 1.0 ml, 1.5 mg/ml lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery

1703352 [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [34.228.30.69, 10.232.2.19] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [if-modified-since] = [Wed, 26 Sep 2018 01:41:45 UTC] [seourl] = [/en-us/sku/1703352-transfectin-lipid-reagent-5-ml] [x-amzn-trace-id] = [Root=1-5c1309bf-0791dd5f85a8cf46932b237d] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1703352] [host] = [10.232.0.21:1776] [x-request-uri] = [/en-us/sku/1703352-transfectin-lipid-reagent-5-ml] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 1703352-transfectin-lipid-reagent-5-ml LSR en US en-us /prd 07/30/13 03:25 AM en_US true internet/generic 2018-04-02 20:17:12 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/20 05:00 PM 1518242136325 2018-04-02 20:17:12 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the high school or college level, visit our <a href="/en-us/education/education-discount-policy?ID=MRFKWO4VY">Education Discount Policy</a> page to establish an education account number.</p> </div> /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE N 0 1703352 09/21/09 12:05 PM N en 09/21/19 12:05 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:55:03 1703352-transfectin-lipid-reagent-5-ml 1703352 TransFectin Lipid Reagent TransFectin™ Lipid Reagent, 5 ml 5 x 1.0 ml, 1.5 mg/ml lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery 5 x 1.0 ml, lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery 5 x 1.0 ml, 1.5 mg/ml lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery /webroot/web/images/lsr/products/gene_transfer_rnai/sku_thumb/global/170-3352_tn.jpg /webroot/web/images/lsr/products/gene_transfer_rnai/sku_view/global/170-3352_view.jpg /webroot/web/images/lsr/products/gene_transfer_rnai/sku_feature/global/170-3352_feature.jpg TransFectin™ Lipid Reagent, 5 ml RepEx 170-3352 5 x 1.0 ml, lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery 1703352, transfectin, lipid, reagent, transfection, cationic, lipid-mediated, gene, delivery, plasmid, dna, sifna, shrna, cells, 5x 1.0, ml 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Transfection/Lipid-Transfection N 0 <p>Choose the general purpose TransFectin Lipid Reagent for high efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells. The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.</p> <ul> <li>1 ml is generally sufficient for 125 to 200 cell transfections in 35 mm plates</li> <li>Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability</li> <li>Transfection at culture densities from 40% to 90%</li> <li>Designed to work in media containing serum but can be used in the absence of serum</li> <li>No post transfection media changes required</li> <li>Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene</li> </ul> <p>The <a href="http://biorad-ads.com/transfection_protocols/" target="_blank">Transfection Protocol Online Library</a> contains protocols from a range of sources for transfection using lipids, electroporation and biolistic particle delivery. The library has a comprehensive list of cell lines and primary cultures. Use the information in the library as a starting point for designing protocols for your cells. The <a href="/webroot/web/pdf/lsr/literature/4106254A.pdf" target="_blank">TransFectin Lipid Reagent Instruction Manual</a> provides information about optimization as well as changes in protocols when changing from a different cationic lipid.</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 300px;" border="0"> <tbody> <tr> <td style="text-align: center;" valign="top"><strong>Volume</strong></td> <td style="text-align: center;" valign="top"><strong>Number of Vials</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">0.5 ml</td> <td style="text-align: center;" valign="top">1 x 0.5 ml</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1703350-transfectin-lipid-reagent-0-5-ml?ID=1703350">1703350</a></td> </tr> <tr> <td style="text-align: center;" valign="top">1 ml</td> <td style="text-align: center;" valign="top">1 x 1 ml</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1703351-transfectin-lipid-reagent-1-ml?ID=1703351">1703351 </a></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">5 ml</td> <td style="text-align: center;" valign="top">5 x 1 ml</td> <td style="text-align: center;" valign="top">1703352</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/silentfect-lipid-reagent-for-rnai">siLentFect&trade; Lipid Reagent for RNAi</a> is optimized for delivery of siRNA alone, or cotransfected with a small reporter-plasmid</li> <li><a href="/en-us/product/spectrophotometry/smartspec-plus-spectrophotometer?ID=e12b12ef-e6e2-4ae3-9faa-fd4bfb60ccca">SmartSpec&trade; Plus Spectrophotometer</a></li> <li><a href="/en-us/product/fixable-viability-dyes">Fixable Viability Dyes</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/electroporation">Electroporation</a></li> <li><a href="/en-us/category/biolistic-particle-delivery-systems">Biolistic Particle Delivery Systems</a></li> <li><a href="/en-us/category/nucleic-acid-extraction-purification">Nucleic Acid Extraction &amp; Purification</a></li> <li><a href="/en-us/category/amplification-pcr">PCR Amplification</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>TransFectin Lipid Reagent <a href="/webroot/web/pdf/lsr/literature/Bulletin_2873.pdf" target="_blank">Brochure</a></li> <li>TransFectin Lipid Reagent <a href="/webroot/web/pdf/lsr/literature/4106254A.pdf" target="_blank">Instruction Manual</a></li> <li>Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types <a href="/webroot/web/pdf/lsr/literature/Bulletin_3197.pdf">Application Note</a></li> <li>Transfection Reagent <a href="/webroot/web/pdf/lsr/literature/Bulletin_5343C.pdf" target="_blank">References</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-transfection?ID=LUSONCB9O">Transfection </a></li> <li><a href="http://biorad-ads.com/transfection_protocols/">Transfection Protocol Library</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1703352.html /webroot/web/html/lsr/products/SKUs/autopopulated/1703352.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703352&CartQuote=QT&quantity= 1395 false true false $1,391.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703352&AddToDefaultWishList=true&quantity= true false true 12161500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703352&quantity= 170-3350-MSDS TransFectin™ Lipid Reagent Australia English /webroot/web/pdf/WWMSDS/LSGC/AUS/AUS_ENG_1703350.pdf Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_1703350.pdf Belgium French 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Choose the general purpose TransFectin Lipid Reagent for high efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells. The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.

  • 1 ml is generally sufficient for 125 to 200 cell transfections in 35 mm plates
  • Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability
  • Transfection at culture densities from 40% to 90%
  • Designed to work in media containing serum but can be used in the absence of serum
  • No post transfection media changes required
  • Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene

The Transfection Protocol Online Library contains protocols from a range of sources for transfection using lipids, electroporation and biolistic particle delivery. The library has a comprehensive list of cell lines and primary cultures. Use the information in the library as a starting point for designing protocols for your cells. The TransFectin Lipid Reagent Instruction Manual provides information about optimization as well as changes in protocols when changing from a different cationic lipid.

Packaging Options

Volume Number of Vials Catalog Number
0.5 ml 1 x 0.5 ml 1703350
1 ml 1 x 1 ml 1703351
5 ml 5 x 1 ml 1703352

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Supporting Documentation

Online Applications

Available Translation
TransFectin™ Lipid Reagent  (SDS)gene

Bulletin# 170-3350-MSDS

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