(Discontinued) ChemiDoc™ V3 Western Workflow™ for Midi Gels #17001390


Pkg of 1, complete suite of instruments and consumables to run chemiluminescent western blotting experiments from start to finish. Includes precast midi gels. Uses Image Lab™ Software.

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Use the ChemiDoc V3 Western Workflow for Midi Gels for streamlined electrophoresis and blotting. The versatile ChemiDoc Imaging System can perform a wide range of blotting applications including stain-free technology, chemiluminescent detection, ethidium bromide, SYPRO Ruby, Coomassie and silver stains. This system can easily be upgraded to obtain the expanded fluorescence capability of the ChemiDoc MP V3 Western Workflow (17001389) with the ChemiDoc to ChemiDoc MP Upgrade Kit (12003263).

The western blotting protocol with the V3 Western Workflow for mini gels provides a five-step approach combining traditional blotting techniques with innovative tools, allowing you to quickly check electrophoresis results and blot transfer quality prior to western blotting.

Features and Benefits

  • Efficient workflow — rapid results with the ability to validate each step
  • High performance imaging and analysis — combined sensitivity with comprehensive analysis and documentation
  • Fluorescence imaging capabilities can be extended — as needs and techniques change, the ChemiDoc Imaging System can be upgraded to encompass RGB, far red, and near infrared, with the fluorescence upgrade package
  • Stain-free imaging enabled — obtain a gel image in 5 min without staining and destaining; normalization by total protein
  • Easy to use — intuitive touch-screen user interface, automatic selection of optimal light source by application, auto focus, auto exposure, and preview features

Product Contents

  • 1 ChemiDoc Imaging System (17001401)
  • 1 Blot/UV/Stain-Free Sample Tray (12003028)
  • 1 Trans-Blot Turbo Transfer System (1704150)
  • 1 Trans-Blot Turbo Midi Nitrocellulose Transfer Packs, 10 per pack (1704159)
  • 1 Criterion Electrophoresis Cell (1656001)
  • 1 Immun-Blot® Low Fluorescence PVDF/Filter Paper Sets (1620262)
  • 1 4–20% Criterion™ TGX Stain-Free™ Protein Gels, 10 per pack (4568094)
  • 1 10x Tris/Glycine/SDS Running Buffer, 1 L (1610732)
  • 1 1x Tris Buffered Saline with 1% Casein, 1 L (1610782)
  • 1 2x Laemmli Sample Buffer, 30 ml (1610737)
  • 1 Precision Plus Protein All Blue Standards, 50 applications (1610373)
  • 1 Precision Plus Protein Unstained Standards, 100 applications (1610363)
  • 1 PowerPac™ Basic Power Supply (1645050)
  • 1 Clarity Western ECL Substrate, 200 ml (1705060)
  • 1 Clarity Max Western ECL Substrate, 100 ml (1705062)


  • ChemiDoc V3 Western Workflow for Mini Gels (17001403)
  • ChemiDoc V3 Western Workflow for Midi Gels (17001390)

Related Products

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Supporting Documents

Online Resources

Smart Tray Technology™ Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Autofocus Precalibrated focus for any zoom setting or sample height
Auto-exposure Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding Dynamic; precalibrated and optimized for each application
Touch-screen functionality Multitouch capability (2 points) 12.1" display
Maximum image area (W x H) 21 x 16.8 cm
Detector Cooled CCD, 6 megapixels
Dynamic range >4 orders of magnitude
Illumination sources Trans-UV, 302 nm excitation
Epi-white (requires White Sample Tray)
Trans-blue, 450–490 nm excitation (requires Blue Sample Tray)
Detection Chemiluminescence detector
590/110 nm standard emission filter for protein and DNA gel and blot imaging
Data output 16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight 35 kg (78 lb)
Operating voltage 100–250 V
Operating temperature 10–28°C
Operating humidity 10–85% relative humidity (non-condensing)
Number Description Options

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