iScript™ RT-qPCR Sample Preparation Reagent #170-8898

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100 reactions, 10 ml, contains RNase inhibitors and RNA stabilizers

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Number Description Options
170-8898-MSDS iScript RT-qPCR Sample Prep Reagent English

Packaging Options

  • Catalog #170-8898, 100 reactions, 10 ml, contains RNase inhibitors and RNA stabilizers
  • Catalog #170-8899, 500 reactions, 5 x 10 ml, contains RNase inhibitors and RNA stabilizers

Description

iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis, RNA stabilization, and removal of genomic DNA for sensitive qPCR without traditional RNA sample preparation.

Key Features and Benefits

  • Have ease-of-use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis, RNA stabilization, and genomic DNA removal in 5–10 min
  • Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
  • Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
  • Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
  • Use with different cell types — compatible with both suspension and adherent cell cultures