Ligation And Transformation Module #1665015EDU

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Kit contains sufficient materials for 12 student workstations (2–4 students per workstation)  
T4 DNA ligase, 10 µl 1
Ligation reaction buffer, 2x, 100 µl 1
Proofreading DNA polymerase, 10 µl 1
pJET1.2 blunted cloning vector, 10 µl 1
BgIII restriction enzyme, 55 µl 1
Restriction enzyme buffer, 10x, 100 µl 1
C-Growth medium, 30 ml 1
Transformation reagent A, 5 ml 1
Transformation reagent B, 5 ml 1
IPTG ,1 M, 0.1 ml 1
Sterile water, 1 ml 1
Colored microcentrifuge tubes, 2.0 ml 120
Microcentrifuge tubes, 1.5 ml 30
Curriculum, including teacher's guide, student manual, and graphic quick guide 1
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