ProteoMiner™ Sequential Elution Small-Capacity Kit #1633010

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Overview

10 preps, protein preparation kit for sequential elution into 4 fractions, for 10 mg of total protein, includes 10 spin columns, buffers, collection tubes
Safety Data Sheet

Safety Data Sheet

Description

Description

Choose the ProteoMiner Sequential Elution Small-Capacity Kit to simultaneously concentrate low-abundance proteins and lower the concentration of high-abundance proteins, and fractionate proteins within the enriched population by sequential elution. This kit is designed for 10 mg samples. Protein complexity of the sample is maintained.

The enrichment protocol uses a large, highly diverse library of combinatorial peptide ligands bound to beads. After high-abundance proteins have saturated all their high-affinity ligands, the unbound excess protein is washed away. Most medium- and low-abundance proteins will not saturate their ligands, resulting in no excess to be washed away. After washing the ProteoMiner Sequential Elution Reagents can then be used to fractionate the retained proteins. The elution kit contains four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics.

  • Compatible with a wide range of sample types
  • Four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics
  • Can be used for differential expression analysis
  • Sequential elution has been optimized for samples for SELDI; eluted fractions are not compatible with direct use of fractions in 2-D gel electrophoresis

Kit Contents

  • Spin columns, 10; 500 µl bead slurry (4% beads, 20% v/v EtOH), 20 µl settled bead volume
  • Wash Buffer, 150 mM NaCl, 10 mM NaH2PO4, pH 7.4, 50 ml
  • Elution Reagent, lyophilized 8 M urea, 2% CHAPS, 2 vials
  • Rehydration Reagent, 5% acetic acid, 5 ml
  • Elution Reagent 1, 1 M sodium chloride, 20 mM HEPES, pH 7.4, 5 ml
  • Elution Reagent 2, 200 mM glycine, pH 2.4, 5 ml
  • Elution Reagent 3, 60% ethylene glycol, 5 ml
  • Elution Reagent 4, 33.3% 2-propanol, 16.7% acetonitrile, 0.1% trifluoroacetic acid, 5 ml
  • Plasma Preparation Buffer, 1 M sodium citrate, 20 mM HEPES, pH 7.4, 1 ml
  • Capless 2 ml centrifuge tubes, 20
  • Capped 2 ml centrifuge tubes, 40
  • Instruction Manual
  • Quick Start Guide

Product Options

ProteoMiner Protein Enrichment and Elution Kits Capacity Number of Samples Catalog Number
Small-Capacity Enrichment Kit ≥10 mg 10 1633006
Introductory Small-Capacity Enrichment Kit ≥10 mg 2 1633008
Large-Capacity Enrichment Kit ≥50 mg 10 1633007
Introductory Large-Capacity Enrichment Kit ≥50 mg 2 1633009
Sequential Elution Reagents   10 1633003
Small-Capacity Elution Kit (combines 1633006 and 2633003) ≥10 mg 10 1633010
Large-Capacity Elution Kit (combines 1633007 and 2633003) ≥50 mg 10 1633011

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Related Categories

Supporting Documentation

  • ProteoMiner Protein Enrichment and Sequential Kit Protocols
  • ProteoMiner Sequential Elution Kit Protocol
  • ProteoMiner Protein Enrichment Kits Instruction Manual
  • ProteoMiner Protein Enrichment Technology Brochure
  • Enrichment of Medium- and Low-Abundance Proteins in Sample Types Using ProteoMiner Technology Application Note